质谱可以和气相联用如GC/MS,也可以和高效液相色谱联用如HPLC/MS。由于色谱和质谱灵敏度相当,再加上分离效果很好的色谱可以作为质谱的进样系统,质谱作为色谱的鉴定仪速度快,分离好,应用广。色谱-质谱联用成为最好的用于分析微量有机混合物的仪器。 在1970年后,质谱-质谱法(massseparetion-massspectraCharacterization...
样品种类实验风险:过表达细胞样品<细胞样品<动物组织样品; CoIP-MS风险:由于存在特异性抗体高丰度蛋白,质谱不一定能够鉴定到诱饵蛋白。【我们的数据确实检测不出诱饵蛋白,不理解这里的解释】 也可以用于检测两组样品间的蛋白(如histone)的修饰差异 To define the impact of chemical disruption of HDAC1/2 on global ...
print("END IONS",file=out_file)print("\n",file=out_file)print("Parsed{0}spectra from file{1}".format(n,args.in_mzML))out_file.close() 运行以后,得到的是下面的结果 BEGINIONSTITLE=Spectrum4,MSlevel2,RT0.02PEPMASS=452.26035CHARGE=2+SCANS=4RTINSECONDS=0.015386716SEQ=63.2951020.6639663.8831009...
Developing an LC-MS-MS spectra library of drugsLCGCSpectroscopy
The Institute of Toxicology in the Clinical Toxicology and Poison Information Centre Berlin (BBGes) and Geneva Bioinformatics (GeneBio) have announced a collaboration to create a new spectral library taking advantage of high mass accuracy data.LCGC Editors...
By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection. Due to its setup, the Orbitrap mass analyzer is actually a Fourier Transform mass analyzer analog of FT-ion cyclotron r...
在代谢组学研究中,GC-MS和LC-MS是两种比较常用的检测平台,Web of Science 的数据显示,LC-MS是目前最主要的代谢组学分析平台。目前医学代谢组学的研究大多集中在临床医学、药理分析领域,而其试验样本大多为人体尿液、血浆、细胞、组织等,这些样本中的代谢...
By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection. Due to its setup, the Orbitrap mass analyzer is actually a Fourier Transform mass analyzer analog of FT-ion cyclotron r...
在代谢组学研究中,GC-MS和LC-MS是两种比较常用的检测平台,Web of Science 的数据显示,LC-MS是目前最主要的代谢组学分析平台。目前医学代谢组学的研究大多集中在临床医学、药理分析领域,而其试验样本大多为人体尿液、血浆、细胞、组织等,这些样本中的代谢物大都能较好地电离。LC-MS灵敏度较高,能分离上千种小分...
By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection. Due to its setup, the Orbitrap mass analyzer is actually a Fourier Transform mass analyzer analog of FT-ion cyclotron resonance (ICR) ...