LC-MS的全称是液相色谱-质谱联用(Liquid Chromatography-Mass Spectrometry),或称为液相色谱-质谱联用仪(Liquid Chromatography-Mass Spectrometer)。它是将液相色谱(LC)的高效分离能力与质谱(MS)的高灵敏度和高特异性分析能力相结合的一种分析技术,广泛应用于化学、生物学、医药和环境科学等领...
LC-MS/MS用于洗脱的缓冲液是水和乙腈的混合物(70/30,v/v,含 0.5% FA),在快速真空蒸发之前,先用 200 μL 进行两步洗脱。用30 μL 胰蛋白酶消化液再悬浮。在 37°C 下过夜消化蛋白质。然后,将 20 μL 提取的样品注入 LC-MS。 5 LC-MS/HRMS色谱条件 色谱柱:在线样品净化柱(Strata-X; 20 x 2.0 ...
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请问LC-HRMS是什么意思啊？和LC-MS是一样的吗？
2.8. LC-HRMS/MS settings The LC-HRMS/MS system consisted of a Thermo Fisher Scientific (TF, Dreieich, Germany) Q-Exactive MS equipped with a heated electrospray ionization (HESI)-II source coupled to a TF Dionex UltiMate 3000 RS LC system comprising a degasser, a quaternary pump, and an ...
LC-HRMS-MS-Qtof Phenolic compounds Acetylcholinesterase HMG-CoA reductase Bioavailability Abbreviations AD Alzheimer's disease AChE acetylcholinesterase HMGR 3-hydroxy-3-methyl-glutaryl-CoA reductase Tris Tris(hydroxymethyl)-aminomethane buffer DTNB 5,5′‐dithiobis‐(2‐nitrobenzoic acid) DPPH 2-diphenyl-1...
The increasing amount of accurate metabolome data that can be acquired on massive sample sets, notably through data-dependent LC-HRMS/MS and NMR profiling, allows the mapping of natural extracts at an unprecedented level of precision. Most progress made recently in accelerating metabolite ...
本研究描述了在两个独立实验室中进行的两种经过验证的方法,一种是使用三重四极杆串联质谱 (LC-MS/MS) 开发的,另一种是使用轨道阱系统 (LC-MS/HRMS) 开发的高分辨率质谱。两种方法都使用全长稳定同位素标记的 nivolumab 样(精氨酸 13C6-15N4 和赖氨酸 13C6-15N2)作为内标。样品制备基于 IgG 免疫捕获,然后...
An automated online-SPE-LC-HRMS/MS method for the sensitive and accurate determination of PB, PHT, CBZ and CBZE in human plasma has been developed. The online analysis allowed the operating time of 13 min. It offers a number of advantages, such as rapid sample preparation (direct sample in...
Targeted isolation of sesquiterpene lactone dimers from Aucklandia lappa guided by LC-HRMS/MS-based molecular networking Phytochemistry Volume 206, February 2023, Page 113557 Purchase options CorporateFor R&D professionals working in corporate organizations. Academic and personalFor academic or personal use ...