Nucleic acid was labeled using DAPI. B, C C9 and GC iPSCs were differentiated into motor neurons. C9-ALS and GC iPSC-MNs were either left untreated or treated with sodium arsenite (500 μM) for 1 hr; where indicated cells were let to recover in drug-free medium for 2 hrs ...
Cholinergic inputs depolarize Martinotti cells.aGFP-labeled SOM + interneurons in a GIN mouse (scale bar 200 µm).bDigital reconstruction of a GFP-expressing MC.cTop trace: Action potential profile of a L5 MC in a GIN/Chat-ChR2-EYFP mouse in response to somatic current injection (...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder involving both upper motor neurons (UMN) and lower motor neurons (LMN). Enormous research has been done in the past few decades in unveiling the genetics of ALS, successfully
In the 9 patients in whom the MSCs were labeled with ferumoxides, MRI of the brain and whole spine was performed at 24 to 48 hours and at 1 to 3 months after injection of MSCs. Hypointense signals in T2-weighted images, indicating the presence of ferumoxides-positive cells, were ...
Two-photon in vivo calcium imaging is a powerful tool to study cortical hyperexcitability in awake animals. Specifically, different types of neurons can be labeled by the expression of calcium indicators, which have a high detection rate for single action potentials [71]. In awakehSOD1G93Amice...
ALS mouse models, including the h SOD1G93A and FUSR521C models, have revealed pre-symptomatic spine loss in the motor cortex [18] and sensorimotor cortex [43], respectively. Intriguingly, in the h SOD1G93A model, early pre-symptomatic loss of spines on pyramidal cells in the...
The LCR images contained craniofacial bones, teeth, and C1-C5 of the cervical spine, which can provide more information about the development and ageing than IPR and DPR images. As shown in Fig.1a, we labeled several instances in LCR images according to the anatomical structure to quantitatively...
BDA-labeled projection neurons varied widely in the shape and size of their cell somas, with mean cross-sectional areas ranging from 60-340 mm . Labeled projection neurons supported 7-55 dendrites that spanned up to 300 mm in length and formed dendritic arbors with cross-sectional areas of ...
silver grains, as detected by light microscopy, was used to determine the tissue distribution of the125I-labeled VEGF165. 4. Generation and Characterization of Transgenic Mice Transgenic mice expressing Flk-1 specifically in adult neurons were generated using the mouse Thy1.2 expression cassette, as...
identification and isolation of particular gene sequences. It is contemplated that any probe used in the present invention will be labeled with any “reporter molecule,” so that is detectable in any detection system, including, but not limited to enzyme (e.g., ELISA, as well as enzyme-based...