This research provides novel insights into the role of drinking water in disseminating ARGs to the gut and strongly suggests that drinking water may also play a major role apart from other factors known to be involved in the prevalence of last resort ARGs in the gut....
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Using shotgun metagenomics, we found a wide array (n聽=聽175) of horizontally transferable antibiotic resistance genes (ARGs) including carbapenemases such as NDM, VIM, KPC, OXA-48 and IMP types. The relative abundance of total ARGs was 30-fold higher in river sediments within the city ...
E. coli clinical isolates carrying ARGs of severe clinical concern were then used to assess the robustness of CRISPRi under different growth conditions. Meropenem, colistin and cefotaxime susceptibility was successfully increased in terms of MIC (up to >4-fold) and growth delay (up to 11h) in ...
A set of three test strains was investigated: sensitive strain, without ARG-carrying plasmids; resistant strain, with the target ARG(s); specific CRISPRi transformant (henceforth sCRISPRi), bearing the ARG-carrying plasmid(s) and a CRISPRi platform targeting one or two ARGs (Supplementary ...