An enzyme-linked immunosorbent assay screen was performed against the immunizing antigens conjugated to BSA and positive clones were expanded in 24-well plates. Hybridomas clones UMO 1 were selected after confirmation of their affinity by enzyme-linked immunosorbent assays conducted against unconjugated ...
the transferrin recycling assay was used as described previously [42]. Briefly, HeLa cells expressing either wild-type FIP5-GFP or FIP5-GFP-P652L were incubated with transferrin conjugated to Alexa488. Cells were then washed and incubated with serum-supplemented media for varying amounts of time...
To that end, we next tested whether Rab11FIP5-P652L has an effect on recyc- ling of transferrin receptors in HeLa cells and found that the P652L substitution did not alter recycling (Additional file 11: Figure S10H). Thus, so far we were unable to find any functional consequences of ...
the transferrin recycling assay was used as described previously [42]. Briefly, HeLa cells expressing either wild-type FIP5-GFP or FIP5-GFP-P652L were incubated with transferrin conjugated to Alexa488. Cells were then washed and incubated with serum-supplemented media for varying amounts of time...