The long and short AMPAR subunits appear to serve different functions in AMPAR surface expression, and a subunit-specific AMPAR trafficking model has been proposed to explain how LTP is expressed and maintained. To summarize, the forward trafficking characteristics of the long-tail GluR1 subunit...
Mass spectrometry and nano-LC settings for the spectral counting experiment were the same as described in detail above (see “Label-free Shotgun Analysis by 1-D SDS-PAGE and Nano-LC-LTQ-Orbitrap Mass Spectrometry of Seedlings”). Quantification of clpr2-1/wt ratios was similar to the ...
The formation of macromolecular complexes can be measured by detection of changes in rotational mobility using time-resolved fluorescence anisotropy. However, this method is limited to relatively small molecules (~0.1–30 kDa), excluding the majority
Therefore, while structures such as the Golgi apparatus, nuclear envelope, mitochondria and the plasma membrane have different biological functions and ultrastructure, they end up in the same endmember class. Incorporation of additional elements, with affinity to specific features - such as lead, ...
Electron paramagnetic resonance (EPR) can be used to measure crude long-distance interactions on the basis of a spin label covalently bonded to the RNA of interest. The method is in its infancy but can provide information for challenging systems, where other structural methods fail. In analogy ...
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The quality of seriation can also be measured by the bandwidth and the envelope of the symmetric similarity matrix. The bandwidth is defined to be the maximum width of all rows (with the row width defined to be the largest distance between any non-zero element within the row and the ...
(N 2 A) using label-free quantitative mass spectrometry. This confirmed a highly specific N-G3BP1/2 interaction fully dependent on an intact ΦxFG motif (Fig.2i, Supplementary Fig.3cand Supplementary Data4). Using a similar approach, we quantitatively compared the interactomes of the N ...
"Expression of Large Hepatitis B Envelope Protein Mutants Using A New Expression Vector", Arch. Virol. 122:367-371 (1992). Falkner et al. "Escherichia coli gpt Gene Provides Dominant Selection for Vaccinia Virus Open Reading Frame Expression Vectors", J. Virol. 62(6)1849-1854 (1988). ...
and that from the small dish is used to label RNA in the viral particles to analyze ratio of packaged replicon and helpers' genomes. The sample with the best ratio (˜1:1:1) will be used for the next passage at MOI used for its preparation. After these detailed experiments, the most...