Ribosomal complex of the large and small subunits of ribosomes which are particulate; an adhesin of a microbe or antigen of a virusdoi:US20040115210 A1The present invention relates to an Immunogenic Complex comprising Ribosomal Complex and Adhesion of a Microbe or Ribosomal Complex and a viral ...
Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore,...
The 55 S mammalian mitochondrial ribosomes consists of small (28 S) and large (39 S) subunits (1). In contrast to bacterial ribosomes which are about 65% RNA, mammalian mitochondrial ribosomes are only 33% RNA. The low percentage of RNA in these ribosomes reflects a reduction in the ...
Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes. Short base-paired RNA fragments, and fragments containing intra-RNA cross-links, were isolated from E. coli 23S rRNA or 50S ribosomal subunits by two-dim...
A new cytochrome a and b deficient nuclear mutant of Neurospora crassa, cyt-U-28, is defective in the assembly of large subunits of mitochondrial ribosomes. Nonetheless, this mutant overproduces apparently normal small subunits of mitochondrial ribosomes, even though it should be deficient for the...
Increasing numbers of protein interactions have been identified in high-throughput experiments, but only a small proportion have solved structures. Recently, sequence coevolution-based approaches have led to a breakthrough in predicting monomer protein s
作者: Yuji Suzuki and Amane Makino 摘要: Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome. For synthesis of the Rubisco holoenzyme, both genes need to be expressed coordinately. To ...
Delayed rRNA processing and up-regulation of a chloroplast DEAD box RNA helicase and polynucleotide phosphorylase, but no significant change in accumulation of ribosomal subunits, suggested a bottleneck in ribosome assembly or RNA metabolism. Strong up-regulation of a chloroplast translational regulator ...
EIF4B, which scored 3.82/5, initiates translation in the cytoplasm by binding RNA substrates and recruiting ribosomes. We hypothesize that this mechanism could drive a false positive when artificially driven to nuclear pre-mRNA in our tethering system, as the mechanism of spliceosome recruitment is...
more than one donor–acceptor pair is necessary, requiring several samples. However, this is a relatively fast method and usually requires a small amount of sample. FRET is most often used on ribosomes, but will likely have large use in the localization of regulatory RNA on genomic DNA or ...