Nucleoli are sites where both the large and small ribosomal subunits mature. Biochemical assays have suggested that a multivalent nucleolar protein, NPM1/nucleophosmin contributes to the formation of the outer layer of the nucleolus. Prior works show that NPM1 depletion disorganizes the nucleolar ...
During translocation, the tRNAs transiently occupy intermediate positions on both small (30S) and large (50S) ribosomal subunits. How EF-G and GTP hydrolysis control these movements is still unclear. We used fluorescence labels that specifically monitor movements on either 30S or 50S subunits in ...
On the other hand, faster HDX was observed for subunits implicated in tRNA translocation between sites or those involved in pivoting of large and small ribosomal subunits. These functions presumably require higher flexibility. Aggregation, amyloid and inclusion bodies Fluids in cells contain ...
although less popular, variant that uses 5S ribosomal RNA (rRNA) as a scaffold was also developed [40]. In the Ponchon and Dardel method, tRNA (human tRNALys3orE. colitRNAMet)
Based on previous studies35,47, both bL19 and bS20 assemble early during the biogenesis of the large and small subunit, respectively. Hence, this setup allowed monitoring and quantification of potential precursors of both the 50S and 30S ribosomal subunits. For precursors of the 30S subunit, ...
et al. Kinetics of spontaneous and EF-G-accelerated rotation of ribosomal subunits. Cell Rep. 16, 2187–2196 (2016). Article CAS PubMed Google Scholar Wang, L. et al. Allosteric control of the ribosome by small-molecule antibiotics. Nat. Struct. Mol. Biol. 19, 957–963 (2012). ...
Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes. Short base-paired RNA fragments, and fragments containing intra-RNA cross-links, were isolated from E. coli 23S rRNA or 50S ribosomal subunits by two-dim...
The stepwise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the pre-rRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many ...
small subunitcytochrome b COBCOBExtensive investigations on apicomplexan mitochondria, such as those of Plasmodium falciparum, revealed that ribosomal RNAs (rRNAs) are fragmented into multiple short pieces. In this study, we isolated three mitochondrial large subunit rRNA (mtLSU rRNA) fragments from ...
This study addresses that need by employing a large-scale comparative proteomics analysis to examine the downstream effects of Plk1 inhibition using the small-molecule inhibitor BI 6727. BI 6727 (Volasertib) is a second-generation Plk1 inhibitor and is currently the most specific Plk1 ATP-...