BF2567EF.CMV.RFP(EFCMVRFP)质粒载体 BF2568EF.PGK.GFP(EFPGKGFP)质粒载体 BF2569EGFP质粒载体 BF2570EMBL12质粒载体 BF2571EMBL3 AamBam(EMBL3AamBam)质粒载体 BF2572EMBL3-cos-Not(EMBL3cosNot)质粒载体 BF2573EMBL3cos质粒载体 BF2574Emerald GFP(EmeraldGFP)质粒载体 BF2575EosFP质粒载体 BF2576EYFP质粒...
Cells were fixed and stained with GM130 antibody to visualize the presence of LAMP1-GFP in Golgi. To monitor the acquisition of LAMP1 by WT or sipC−:SCP from Golgi-derived vesicles, RAW 264.7 macrophages overexpressing LAMP1-GFP were infected with RFP:WT or RFP:sipC− Salmonella for 5...
Stable cell lines with fluorescent nuclei or fluorescent F-actin were generated through transduction with lentiviruses expressing H2B-GFP (Addgene #25999), H2B-RFP (Addgene #26001) or LifeAct-mRuby. Transient knockdowns were done by transfection of small interfering RNAs (siRNAs) into cells ...