工作原理:BLOCK-iT™ RNAi 靶标筛选系统方法使用 β-半乳糖苷酶活性试验测量 RNAi 试剂(Stealth RNAi™ siRNA、siRNA、dicer 库或含 shRNA 的质粒)的敲低能力。要确定对您的靶标极有效的敲低试剂,只需将您的靶基因克隆到 pSCREEN-iT ™/lacZ-DEST Gateway™ 载体中即可。将所得构建体与正在检测的敲...
Sequence conversiongene correctionsingle-stranded DNA fragment5'-tailed duplexgenome editingTargeted DNA editing has great potential to cure some genetic diseases; however, the use of artificial nucleases such as CRISPR-Cas9 and TALEN in gene therapy can potentially cause severe side effects due to ...
In vitro infection revealed that the AFP but not the IGFII transcriptional regulatory sequence controlled nls lacZ expression specifically in hepatoma cells. ... PB Arbuthnot,MP Bralet,CL Jossic,... - 《Human Gene Therapy》 被引量: 225发表: 1996年 AFP(+), ESC-derived cells engraft and dif...
Here, we show trans-conjugation of pGEM-T cloning vector (modified Promega PCR product cloning vector with tra genes, transposable element (Tn5)) and M13 sequence via α-complementation mechanism in order to activate β-d-galactosidase gene in DH5α strain (non-lactose-fermenting host). Results...
Bovine alpha S1-casein gene sequences direct high level expression of active human urokinase in mouse milk [J].Biotechnology,1990,8(5):443-446. [5]Riego E,Limonta J ,Aguilar A,et al. Production of transgenic mice and rabbits that carry and express the human tissue plasminogen activator ...
MAR: Matrix attached regions (MARs) are sequences typically AT-rich that are able to form barriers between independently regulated domains [2]. pCpG plasmids contain two MARs, from the 5’ region of the human IFN-β gene or β-globin gene that were chosen because they are naturally CpG-fr...
1996: Isolation, sequence, and expression of the mouse oxytocin receptor gene Pediatric Research 39(4 Part 2): 68A Jin, L.; Jin, W.-l.; Ju, G. 2004: Cloning of the complete coding sequence of mouse oxytocin receptor gene and its eukaryotic expression Xi Bao Yu Fen Zi Mian Yi Xue ...
eukin-2andporcineinterleukin-6eDNAsequenceswereclonedintotheexpressing vectorspET-28aandpGEX-KGrespectively.TheywereexpressedinE.coliBL21(DE3)withhigh-levelpro。 duetion.ThegenedeletedvaccineofpseudorabiesvirusEastrain(TK-/gG-/LacZ十)wasmixedwiththetwo differentpurifiedrecombinan tproteinseach,orboth,withth...
Human alpha lactalbumin was detected at 72 h after transfected too. The production of human alpha lactalbumin was 0.64 g/L approximately. The results indicate that the bovine mammary epithelial cell line have the ability of expressing exogenous gene, the 5' flanking sequence of bovine alpha S1 ...
Wang Q, Zhang G, Du YH, Zhao Y, Qiu GY (2003) Low-energy 30 keV carbon ion induced mutation spectrum in the LacZ Gene of M13mp18 Double-Stranded DNA. Mutat Res 528:55–60Wang Q,Zhang G,Du Y, et al.Low-energy (30 keV) carbon ion induced mutation spectrum in the LacZ alpha ...