Bacterial trans-conjugation was used as a tool to activate β-galactosidase productivity through alpha complementation between pGEM harboring E. coli LK111 and recipient E. coli DH5α (∆M15lacZ). Active trans-conjugant isolates further improved its productivity through UV mutagenesis, and M-KH-...
plasmid vectors for lacZ alpha-complementation High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol- or kanamycin-resistance selection 上传者:u012769258时间:2013-11-08 CLONTECH酵母双杂中文版.doc ...
Trieu-Cuot P, Carlier C, Poyart-Salmeron C, Courvalin P. Shuttle vec- tors containing a multiple cloning site and a lacZ alpha gene for con- jugal transfer of DNA from Escherichia coli to gram-positive bacteria. Gene 1991; 102:99-104....
Lee BU, Hong JH, Kahng HY, Oh KH. 2006. Construc- tion of an Escherichia-Pseudomonas shuttle vector con- taining an aminoglycoside phosphotransferase gene and a lacZ´´ Gene for alpha-complementation. J Microbiol 44:671-673.Lee, B.U., Hong, J.H., Kahng, H.Y., Oh, K.H., ...