Scanning along the DNA for bound proteins was achieved using the automated microscope stage to move the tightrope with the nanoprobe contacting the DNA. Scans were performed multiple times on the same DNA tightrope in either the x or y dimensions (depending on the orientation of the DNA). To ...
Optical layout of the experiments. The instrument is based on a Nikon Eclipse Ti confocal microscope. A Ti:Sa laser operating in CW mode provides the 810-nm radiation that enters through one of the rear ports of the microscope and is reflected by a dichroic mirror. The infrared spot position...