We recommend performing the labeling procedure using the Control HeLa RNA included in the system to determine the efficiency of the labeling reaction. The section onFirst-Strand cDNA Synthesisdescribes how to set up the control reaction andAssessing Labeling Efficiencyhas...
The section on First-Strand cDNA Synthesis describes how to set up the control reaction and Assessing Labeling Efficiency has equations for calculating the efficiency of the labeling procedure. TOP Materials Kit Sizes and Modules All versions of the SuperScript™ ...
Wear latex gloves while handling reagents and RNA samples to prevent RNase contamination from the surface of the skin. Use proper microbiological aseptic technique when working with RNA. Dedicate a separate set of pipettes, buffers, and enzymes for ...