We have successfully cleared multiple human organs, including kidney, pancreas, heart, lung, spleen and brain, as well as hard tissue like skull. We also describe an advanced volumetric imaging system using a commercial light-sheet fluorescence microscope that can accommodate most human organs and ...
bone sections2. Notably, such conventional methods obscure specific cellular structures, e.g., neural innervations, that would be better observed with the intact, unsectioned bones. Moreover, histological sectioning could be challenging for many bone tissues, e.g., vertebral column, skull and ...
Understanding the structural organization of organs and organisms at the cellular level is a fundamental challenge in biology. This task has been approached by reconstructing three-dimensional structure from images taken from serially sectioned tissues,