structuresarealsorevealedatthelevelofindividualcellsandneuronprocesses.Notonlydoesthisallowthephysicalrelationshipsamongintracellularlylabeledneuronstobede-terminedbyreferencetospecificfeaturesintheneuropilbutitalsoenablesafunctiontobeascribedprovisionallytoparticularregionsofneuropil.Thesemethodshaveparticularutilityformapping...
Image analysisMatlabSkeletonThe spatial distribution of synaptic inputs on the dendritic tree of a neuron can have significant influence on neuronal function. Consequently, accurate anatomical reconstructions of neuron morphology and synaptic localization are critical when modeling and predicting physiological ...
Expansion microscopy (ExM) enables imaging of preserved specimens with nanoscale precision on diffraction-limited instead of specialized super-resolution microscopes. ExM works by physically separating fluorescent probes after anchoring them to a swellable gel. The first ExM method did not result in the ...
Six and twenty-month-old female, albino Swiss mice were housed, from weaning, in a standard or enriched environment, including running wheels for exercise and tested for object recognition and contextual memories. Young adult and aged subjects, independent of environment, were able to distinguish fa...
Astrocytes, a subtype of glial cells with a complex morphological structure, are active players in many aspects of the physiology of the central nervous system (CNS). However, due to their highly involved interaction with other cells in the CNS, made pos
注:的短寡核苷酸序列LIM-4 / SOX-2探头是5'Dye-GGGAGAAGATTTTG。 5'Dye标记短寡核苷酸的最小合成规模为100纳米。因此,寡核苷酸需要HPLC纯化。 重悬的寡核苷酸在1×的Tris-EDTA缓冲液(TE:10mM的Tris-盐酸,pH为8.0; 1mM EDTA)中,以100μM的最终浓度。 退火短(5'Dye)和长寡核苷酸。 混合0.6 5'Dye-...
On the one hand the projection area, surface, number, and density of branches determine which and how many other neurons can form synaptic connections with a neuron. On the other hand dendritic structure has profound effects on the computation of synaptic input. This chapter describes a tool ...
The combination of specific fluorescence labeling of functionally characterized neurons and confocal laser scanning microscopy (CLSM) opens the possibility of directly correlating the 3-D morphology with the function of the neuron under study. This paper describes the application of the CLSM technique to...
Here, we use TIRFM (total internal reflection fluorescence microscopy) to image the trafficking of E6BSA-FITC, and GFP-labeled ERα, in live cells in real time. We show that activation of plasma membrane ERs by E6BSA-FITC result in internalization of the fluorescent ligand in live N-38 ...
Iron oxide labeled Clostridium novyi-NT spores for image-guided bacteriolytic therapyMotor neuron cell death represents a pathological feature common to both sporadic and familial (FALS) forms of amyotrophic lateral sclerosis, including those caused by mutations in superoxide dismu…" [more]doi:10.1016...