The Venn diagram (Fig. 1B) shows that FASP is rather complementary to in-solution digestion as the overlap of quantified proteins was only 61%. We also tested the reproducibility of protein quantification using the LFQ intensity values. Both methods performed equally well with an average ...
Quantification data represent results obtained from three independent experiments. ChIP assay was performed in OP9-DL1 cells without treatment (d) and treated with 0.2 ng/mL TGF-β1 or vehicle for 6 h (e). Chromatin was immunoprecipitated with antibodies against Smad 2/3, HDAC1 and H3K14ac,...
and lays inside the cavity of synovial joints (Fig.1a). It is a typical biological tissue that includes chondrocytes and collagen fibers of distinct orientations depending on the location within the tissue. As shown in the schematic diagram (Fig.1b) and a representative transmission image (Fig....
Protein Identification and Quantification The raw files from the mass spectrometer were uploaded onto the Progenesis LC-MS (version 4.1, Nonlinear Dynamics, Newcastle upon Tyne, UK). The LC-MS data were normalized and aligned according to the manufacturer's specifications. The Mascot generic file (...
Figure 4. Venn Diagram of threeexperiments 重复相关性分析(Pearson Correlation) 由Basepeak图可见,三次实验Basepeak图具有较高的相似性(图5)。我们用MaxQuant软件分别对泛素化蛋白和泛素化肽段的强度进行分析,计算三次试验之间的pearson相关系数,发现R值均大于0.9(图6,图7),说明三次实验重复性较好[5]. ...
To investigate the differentially expressed proteins in a complex biological system, strategies for reproducible and accurate quantification are required. Several quantitative proteomics methods have been developed, including stable isotope labeling and label-free methods [28]. Although isotope labeling ...
The quantification of membrane-associated biomolecular interactions is crucial to our understanding of various cellular processes. State-of-the-art single-molecule approaches rely largely on the addition of fluorescent labels, which complicates the quant
Quantification of cytoskeleton composition in musculoskeletal cancer lines and corresponding healthy cells. (A) F-actin protein (ELISA) and (B) gene expression (qPCR), (C)β-tubulin protein and (D) gene expression, (E) ARPC 2/3 complex gene expression. Data presented as bar diagram ± ...
enabling rapid detection and quantification of pathological regions. Besides, a novel diagnostic method has been developed by fusing the H&E segmentation results of cell nuclei with multiphoton images, leading to more accurate diagnoses of microinvasion in ductal carcinoma in situ82. The method of co...
used. For peptides the maximum false discovery rate (FDR) was set to 0.01 and 0.05 for proteins. A two minute window for match between runs was set when label free quantification was enabled. Each file was kept separate in the MaxQuant experimental design template, to obtain individual ...