ThT is known to be a light-up probe in the presence of many DNA structures, including several G-quadruplexes (G4)1,2, G-triplex3, bulged, and mismatched structures4,5. Due to its easy availability, water solubility, and convenient excitation and emission wavelength, ThT has been used ...
The synthetic methodology used to prepare the labeling reagents of the invention is described below. The structures of these compounds are included in FIG. 1. FIG.1(a): Preparation of 3-bromo-1-(trifluoroacetamidyl)propane To a solution of 3-bromopropylamine (2.19 g, 10.0 mmol, Aldrich Che...
8. The biosensor of claim 1, wherein the sample handling structure is selected from the group of structures consisting of a microplate with individual sample wells, a microscope slide, a chip or device having microfluidic fluid channels, a test tube, a petri dish, a flask, a tape cassette ...
Low-melting-point agarose was obtained from Fermentas (Burlington, ON, Canada), and the pGEM®-T vector was purchased from Promega (Madison, WI, USA). PCR master mix (2×) and the DNA ladder were purchased from the company Amplicon. Deionized and pyrogen-free water was obtained from a ...