UV irradiation of rat liver F 1 ATPase, previously exposed to Mg 2+ and [β,γ- 32P]-2-azido-ATP and separated from medium nucleotides, covalently modifies two tyrosine residues in adjacent tryptic peptides of the β subunit. This results from the occupancy by 2-azido-ATP or 2-azido-ADP...
It has recently been suggested that cyclic nucleotides play an important role in taste cell transduction in vertebrates. Amakawa et al. [1] found that dibutyryl cyclic GMP (dbcGMP) had a stimulatory effect on the labellar sugar receptor cell of the blowfly; they suggested that cyclic GMP (...
The signals in the range of 300 nm to 400 nm correspond to the adsorption of pyrene and the signals around 280 nm correspond to the adsorption from nucleotides. Based on their extinction coefficients, the conjugation efficiency is ~100%. In order to confirm the aptamers were successfully...
Moreover, this methodology was used for the determination of biomolecules in real serum samples with satisfying results. MicroRNA was a family of small and non-coding RNAs that was 18–25 nucleotides in length1. More and more evidences indicated that miRNA participated in the gene expression and...
Besides, this novel assay has been successfully employed for the enzyme- and label-free fluorescence detection of miRNA from real samples. Introduction MicroRNAs (miRNAs) are a group of small, noncoding single-stranded RNA (ssRNA) with a molecular length of 18–25 nucleotides (nt), which are ...
The PR/RT region (HXB2 nucleotides 2242 to 3312) was amplified by two-round nested PCR, using primer pair of 5′-CAAGGGAAGGCCAGGGAATTT-3′ and 5′-TGACCC-ATCAAAAGACTTAATAGCAGAAATA-3′ in the first round, and 5′-CCT-TTAACTTCCCTCAGGTCACTCT-3′ and 5′-ATTGACAGTCCAGCTGT...
The two modified peptides are identical to the ones modified by 2-azido-adenine nucleotides in the beef heart F 1 ATPase. Both catalytic and noncatalytic sites are labeled when the ATPase is exposed to [β- 32P]-2-azido-ADP in the presence or the absence of 5′-adenylyimidodiphosphate (...
The luminescence of 1 in the presence of different G-quadruplex DNA structures, including sequences containing a 5′ -side loop, a central loop or a 3′ -side loop, with loop sizes rang- ing from 1 to 15 nucleotides (nt) were tested (Fig. 4d–f). The results showed that the...
MicroRNA (miRNA) was a promising class of cancer biomarkers. Here we developed a label-free method for sensitive measurement of let-7d miRNA based on multiple amplification techniques. The primer will bind to the duplex strand DNA that was formed by stem-loop template and target let-7d to in...
detection techniques. This binding event is brought about by a precise molecular alignment and interaction of complementary nucleotides of the probe and target. It is energetically favored by the release of non-covalent bonding free energy, e.g., hydrogen bonding, stacking free energy and the like...