It has recently been suggested that cyclic nucleotides play an important role in taste cell transduction in vertebrates. Amakawa et al. [1] found that dibutyryl cyclic GMP (dbcGMP) had a stimulatory effect on the labellar sugar receptor cell of the blowfly; they suggested that cyclic GMP (...
1625 Nucleotides, lipids, proteins, ν(C = C) olefinic ν, stretching; δ, deformation; ρ, rocking; γT, twisting; γT, wagging; sym., symmetrical; asym., asymmetrical; Tyr, tyrosine; Phe, phenylalanine. A, adenine; T, thymine; C, cytosine; G, guanine. Tentative assignment according ...
of label‑free electrochemical biosensors Shimaa Eissa 1,2,3, Jeanne N'diaye1, Patrick Brisebois1, Ricardo Izquierdo1, Ana C. Tavares 2 & Mohamed Siaj1* The integration of graphene materials into electrochemical biosensing platforms has gained significant interest in ...
Moreover, this methodology was used for the determination of biomolecules in real serum samples with satisfying results. MicroRNA was a family of small and non-coding RNAs that was 18–25 nucleotides in length1. More and more evidences indicated that miRNA participated in the gene expression and...
The luminescence of 1 in the presence of different G-quadruplex DNA structures, including sequences containing a 5′ -side loop, a central loop or a 3′ -side loop, with loop sizes rang- ing from 1 to 15 nucleotides (nt) were tested (Fig. 4d–f). The results showed that the...
Five micrograms of the total RNA was used to produce cDNA, using Random hexamer primers (Hoffmann-La Roche Ltd., Switzerland) under conditions suggested by the supplier. For the PCR, 1 μl of cDNA was used to amplify a region of 266 nucleotides at the protein NS5 with the primers: ...
(Env) expressed on the surface of virus-producing cells. Although the first generation of anti-HIV-1 CAR-T cells, CD4ζ CAR-T cells, showed only modest antiviral efficacy in phase I clinical trials1,2, researchers have improved designs of the CAR, e.g., decorating the CAR with ...
The signals in the range of 300 nm to 400 nm correspond to the adsorption of pyrene and the signals around 280 nm correspond to the adsorption from nucleotides. Based on their extinction coefficients, the conjugation efficiency is ~100%. In order to confirm the aptamers were successfully...
fragments contain increasingly more radioactivity. Further,35S-labelled nucleotides can be used in place of32P-labelled nucleotides, resulting in sharper definition; and the reaction products are simple to interpret since each lane corresponds only to either G, A, T or C. The enzyme used for most...
Yamamoto, Rika and Kumar, Penmetcha K.R., Molecular beacon aptamer fluoresces in the presence of Tat protein of HIV-1, Genes Cells 5:389-396, (2000). Zhu et al., Directly labeled DNA probes using fluorescent nucleotides with different length linkers, Nucl. Acids Res. 22:3418-3422, (...