1a). The channel cross-sectional dimensions can range from tens to hundreds of nanometers, depending on the size of the investigated nano-object, while their lengths typically exceed the microscope’s field of view (Extended Data Fig. 1 and 2; see ‘Nanofluidic chips’ and ‘Experimental ...
An optical microscope employs visible light to unravel the mysteries of the microscopic world. This minimally invasive tool is used by labs across the world, with applications ranging from embryonic imaging, and cell biology to drug research1,2,3,4,5,6. Since its invention, the major challenges...
Click here to watch the interview! How does it work? In 2018Arthur Ashkinwon theNobel Prize in Physicsfor his discovery that the momentum of light can be used as an incredibly sensitive set of “tweezers”. By shooting a laser through a microscope, he created a highly focused beam of ligh...
W. Mickols, M. F. Maestre,Scanning differential polarization microscope: Its use to image linear and circular differential scattering, “Review of Scientific Instruments,” 59:867, 1988. 17. A. Le Gratiet, A. Diaspro et al.,Circular intensity differential scattering (CIDS) scanning microscopy ...
single-cell resolution and microsecond pixel times.Moreover,it can be implemented on a 3P microscope and provides label-free RBC-specific contrast at depths exceeding 600μm in live adult zebrafish brain.Our results establish a new multiphoton contrast extending the palette of deep-tissue microscopy...
After fixation, the Raman spectra of the cells were recorded. For the reduction-oxidation experiment, 10 mM SDT was added to the medium for 10 min, and the reduced form of the cell was observed with a Raman microscope. The Raman spectrum measurements were carried out on at least ten ...
PGP9.5-immunoreactive nerve fibers present in the epidermis of each section were counted under a light microscope at a magnification of 40× (Leica ICC50D, Leica, Germany). Small-animal model of SNI The mice were anesthetized using ketamine (50 mg/kg) and xylazine (15 mg/kg). SNI surgery...
Freeze-fracture is a unique investigative tool for visualization of the en face topography of individual membrane leaflets of cell membranes at high resolution under the electron microscope. The development of a system of freeze-fracture cytochemical and immunocytochemical techniques has further advanced th...
Raw mass photometry images of dynamin on an SLB exhibited an optical background caused by the roughness of the microscope coverslip (Fig. 1a; raw images). By implementing a sliding median background subtraction33, we obtained a nearly shot noise-limited imaging background, revealing diffraction-li...
et al. Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications. Lab Chip 10, 1417–1428 (2010). Article CAS Google Scholar Palik, E.D. Handbook of Optical Constants of Solids. http://www.sciencedirect.com/science/book/...