Raw mass photometry images of dynamin on an SLB exhibited an optical background caused by the roughness of the microscope coverslip (Fig. 1a; raw images). By implementing a sliding median background subtraction33, we obtained a nearly shot noise-limited imaging background, revealing diffraction-li...
This prism allows the acquisition of an image stack consisting of eight z-planes simultaneously. An algorithm is then used to retrieve phase information from the brightfield image stack using Fourier filtering, transforming it into a QPI stack. We used this microscope to collect a dataset of ...
A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scannin... YY Fei,JP Landry,YS Sun...
Electron microscope analysis of seeded assembly Microtubules are polar structures, and this polarity is reflected in their biased directional growth. Following a convention established previously (G. G. ... Bergen,L. G. - 《Journal of Cell Biology》 被引量: 526发表: 1980年 Study of phase ...
The stiffness of living cells was assessed using a CellHesion200 (Bruker, Billerica, Massachusetts, USA) atomic force microscope (AFM) system, mounted onto an inverted light microscope (AxioObserver D1, Carl Zeiss Microscopy, Jena, Germany). A 5 μm radius spherical tip (model: SAA-SPH-5 ...
The surface morphology analysis can be carried out by Scanning Electron Microscope (SEM). After alkali treatment, the surface morphology of fibers has shown the significant changes occurred on the surface of Borassus fruit fibers. The fiber surfaces have gold coated before recording the micrographs (...
Click here to watch the interview! How does it work? In 2018Arthur Ashkinwon theNobel Prize in Physicsfor his discovery that the momentum of light can be used as an incredibly sensitive set of “tweezers”. By shooting a laser through a microscope, he created a highly focused beam of ligh...
FTIR imaging FTIR was conducted with a Cary 670 spectrometer (Agi- lent Technologies), coupled to a Cary 620 microscope (Agilent Technologies) in transmission mode. The micro- scope features a 128 × 128-element focal plane array detector and a 15 × (0.62 NA) objective. In ...
1a). The channel cross-sectional dimensions can range from tens to hundreds of nanometers, depending on the size of the investigated nano-object, while their lengths typically exceed the microscope’s field of view (Extended Data Fig. 1 and 2; see ‘Nanofluidic chips’ and ‘Experimental ...
et al. Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications. Lab Chip 10, 1417–1428 (2010). Article CAS Google Scholar Palik, E.D. Handbook of Optical Constants of Solids. http://www.sciencedirect.com/science/book/...