To tag (a hormone, enzyme, or other substance) with a radioactive tracer. n. A radioactive isotope used as a tracer; a radiotracer. American Heritage® Dictionary of the English Language, Fifth Edition. Copyright © 2016 by Houghton Mifflin Harcourt Publishing Company. Published by Houghton...
Honarmand Ebrahimi1, Peter-Leon Hagedoorn1, Denise Jacobs2 & Wilfred R. Hagen1 Accurate label-free methods or assays to obtain the initial reaction rates have significant importance in fundamental studies of enzymes and in application-oriented high throughput screening of enzyme ...
In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship. Discuss in detail the lock-and-key theory of enzyme-substrate interaction with a diagram and briefly explain the two types of inhibition in enzy...
demonstrated the possibility of using these proteins as potential biomarkers for early detection of RCC, absolute quantification of these proteins, together with the development of an inexpensive high throughput technology, is required to advance these findings. Conventional labeled assays such as enzyme-...
Zhang et al.9 used a glucose sensor by exploiting the enzyme, neuraminidase (NA), and found the limit (102 PFU) and range (102–108 PFU in 1 hr) of detection for this first-generation assay. A boron-doped diamond electrode terminated 1School of Mechanical and Nuclear Engineering, ...
Nanomaterials with enzyme properties possess excellent catalytic activity and stability. We prepared new nanoenzymes to construct a label-free electrochemical immunosensor for the detection of hepatitis B e antigen (HBe Ag). In this study, PdCu tripod (PdCu TP) functionalized porous graphene (PG) ...
The black bar in the second graph indicates the duration of a period of continuous rainfall. 36 Tab. 2: Distribution of Cataglyphis mauritanica nests,... M Knaden,R Wehner - 《Myrm News》 被引量: 3发表: 2005年 Enzyme stabilisation due to incorporation of a fluorinated non-natural amino...
SDA is a simple, isothermal method, which does not require reverse transcriptase enzyme to detect miRNA. In this study, we have developed an SDA method to specifically detect miR-215. This method has several advantages. It is label-free, low-cost, simple, room temperature, isothermal (no ...
(MMP-1)6. Our observations are also consistent with results reported by Kim et al.24; they used immunostaining to characterize the levels of the collagen cross-linking enzyme lysyl oxidase (LOX) within cells, as a quantitative measure of matrix cross-linking level, and they observed an ...
The secondary antibody is an enzyme-conjugated antibody such as alkaline phosphatase or peroxidase showing a specific color reaction due to a specific characteristic added in this type of antibody. However, this ELISA method may be complex due to the use of the secondary antibody, less accurate ...