1034 Words 5 Pages Open Document Sub Aim 2.1. Generate global transcriptional sequencing data for CML and MLL cells to investigate the response of malignant cells to SMYD2 inhibitors. Experimental methods: Molecular analyses and RNA sequencing: We will constructed a total of four libraries for CML...
The results are read by a florescence camera system and the diagnosed RNA/DNA is determined from the captured fluorescent pattern. The company CEO's philosophy was to conduct only RT-PCR as it can amplify both RNA as well as the DNA and there is only one protocol, making the system ...
Since the more complex method showed no additional benefits, the health system updated their ELISA reporting guidelines in 2023 to recommend only the low-heparin OD approach. The researchers then compared HIT test results from two 5-month periods: one before ...
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Results Optical trapping of cancer cells To develop this novel “intelligent” method to simultaneously trap and identify different human cancer cells we first had to fabricate a lensed fiber tip that is able to individually optically-trap such cells with no material contact to minimize cell disturba...
Positive IHC detected in human tonsillitis tissue, mouse spleen tissue, human lymphoma tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human pancreas cancer tissue, rat thymus tissueNote: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen...
B cells stained with isotype-matched anti-Ig (gray peak) show autofluorescence only; anti-BCR-treated B cells kept at 4 °C (blue line) show a high level of BCR fluorescence; anti-BCR-treated B cells transferred to 37 °C (red line) show an intermediate fluorescence, indicating loss of...
The term "association rate constant," "kon" or "ka" as used herein, refers to the value indicating the binding rate of an antibody to its target antigen or the rate of complex formation between an antibody and antigen as shown by the equation below: AntibodyAb+AntigenAg→Ab−Ag....
Ideally, cell culture dishes in those facilities should be completely filled with medium devoid of air bubbles to avoid unspecific results generated by shear stress during the microgravity simulation [45,46]. Dealing with shear stress is time-consuming and impractical due to the troublesome procedure...
Results: 70 patients (pts) (51 female, 19 male) were identified, with a mean age at RAS diagnosis of 53 yrs. Primary diseases were invasive breast cancer (18 cases), Hodgkin lymphoma (14), cervical cancer (6), non-small cell lung cancer (3), endometrial cancer (3), head and neck ...