Cattle: For the treatment of bacterial pneumonia, pasteurellosis (associated with the shipping fever complex) and footrot caused or complicated by bacteria susceptible to oxytetracycline, and for the treatment of pinkeye (infectious bovine keratoconjunctivitis) caused byMoraxella bovis.Swine: For the tr...
Soluble bovine ocular mucins can interact with and stabilize tear lipids (73, 74), but alone lack non-Newtonian behavior at physiological concentrations (73). That tear lipid binding proteins may functionally interact in modules, as suggested via Cytoscape for lacritin with lipocalin-1, ...
These antibodies against exogenous insulins are found in >95% of patients treated with porcine and bovine insulins (1). Although the prevalence has decreased after the introduction of human insulin and insulin analogues, it is still not uncommon to detect these antibodies in insulin treated patient...
Briefly, Hep-2 mammalian monolayers were formed following incubation of 100 ml/well in 96-well plates in antimicrobial- free growth medium consisting of minimal essential medium with 10% fetal bovine serum and 220 m g of L- glutamine/liter added. Cells were centrifuged (1,200ϫg for 1 ...
The digested tissues were separated from white matter lipids and other cellular debris by centrifugation (at 1000g for 20 min) in the presence of 20% bovine serum albumin solution prepared in DMEM. The resultant pellets were further digested in the presence of 10 mg/ml of collagenase/dispase ...
Briefly, cells were fixed in 2% paraformaldehyde and then permeabilized with 0.1% saponin–2% bovine serum albumin (BSA) in PBS. Fc receptors were blocked using CD16/CD32 Fcγ III/II (BD Biosciences, clone 2.4G2). Cells were stained using the following panel of antibodies (all antibodies ...
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In brief, C2C12 cells were purchased from American Type Culture Collection and propagated in DMEM (Gibco) containing 10% heat-inactivated bovine growth serum and supplemented with penicillin-streptomycin (1%) at 37 °C and 5% CO2. We labeled C2C12 cells with membrane-permeant cell trackers (...
Protein concentration was determined using Bradford reagent [23] with bovine serum albumin (BSA) as a standard. 2.5. Inhibition of TNF cytotoxicity for L929 cell line The TNF cytotoxicity for L929 cells was assessed using neutral red staining technique as described in [16], [18]. Briefly, 5 ...
Commonly used carriers which are chemically coupled to the immunizing peptide include, for example, keyhole limpet hemocyanin (KLH), thyroglobulin, bovine serum albumin (BSA), and tetanus toxoid. Antibody production is verified by analyzing a serum sample, removing the spleen to obtain B lymphocytes...