Light microscopes with relatively low magnification can provide valuable information about changes in the size of exposed cells. Electron microscopes enable the observation of external cell features characterizing death by apoptosis or n ecrosis21. It is possible to observe more typical internal cell...
Toxicity evaluation of ZnO nanostructures on L929 fibroblast cell line using MTS assaydoi:10.1063/1.4915162ZnO has wide applications in medical and dentistry apart from being used as optoelectronic devices such as solar cells, photodetectors, sensors and light emitting diodes (LEDs). Therefore, the ...
Both cell types adhere and proliferate on the biomaterial surface maintaining their characteristic morphology. Transitory changes on cell cycle, size, and complexity are observed. The biomaterial induces apoptosis in Saos-2 osteoblasts but not in fibroblasts. A transitory stimulation of fibroblast ...
SeVpi-infected L929 cells showed no CPE and were easily subcultured at 35°C, resulting in establishment of a persistently infected cell line (L929/SeVpi cells). Subsequently, the presence or absence of virus-specific polypeptides was analyzed in L929/SeVpi cells. The cells used in this ...
poration medium, molecular size of the transferred mole- cules, shape of the target cells, charge of the cell membrane as well as the cell density [17–20]. Thus, the optimal electroporation parameters must be determined empirically for each cell line severally. In the present study, we deter...
The viability of cell line upon contact with the surface of nanocrystalline TiO2film was comparable to the control sample. L929 cells had homogenous cytoplasm and were forming a confluent monofilm, while lysis and inhibition of cell growth was not observed. Moreover, the viability in contact with...
There was no detectable reduction in the 4 S size-class of the transfer RNA, but leucine-accepting activity of the tRNA was slightly impaired. (5) Leucine incorporation in response to exogenously added reovirus mRNA catalyzed by a cell-free protein synthesizing system prepared from mouse ascites ...
4.1. Cell Culture The murine fibroblast cell line L929 was purchased from Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany). The cells were cultured in RPMI medium 1640 without or with 15 mg/L methionine (Gibco, Life Technologies; Darmstadt,...