Expression of EGFP-L10a and assays of function in clock cells.Yanmei HuangJoshua A. AinsleyLeon G. ReijmersF. Rob Jackson
We used bacterial artificial chromosome (BAC) transgenic mice expressing eGFP tagged ribosomal protein L10a under the promoter of the Aldh1L1 gene, a highly expressed astrocytic gene. Using this approach, we could extract only the astrocytic mRNAs, and only those already committed to be translated...