L-Rhamnulose 1 -Phosphate Aldolase fromChiut, TehhsingProcedure, ExperimentalHeath, C
l-Rhamnulose 1-phosphate aldolase ( l-rhamnulose 1-phosphate l-lactaldehyde lyase, EC 4.1.2.b) was sensitive to inhibition by the metal chelators, 8-hydroxyquinoline, 8-hydroxyquinoline-5-sulfonic acid, 1,10-phenanthroline, and 2,2-bipyridine. The relative effectiveness of the inhibition ...
This chapter describes the preparation, purification, and crystallization of L-Rhamnulose 1-phosphate. L-Rhamnulose 1-phosphate may be prepared by using two mutant strains of Escherichia coli. E. coli K12 HfrP72 is grown overnight with shaking at 37° in Difco nutrient broth to the ...
L-Rhamnulose-1-phosphate aldolase from Thermotoga maritima in organic synthesis: One-pot multistep reactions for the preparation of imino- and nitrocyclitols. Adv. Synth. Catal. 2015, 357, 1951-1960. [CrossRef]
L-Rhamnulose-1-phosphate aldolase from Thermotoga maritima in organic synthesis: One-pot multistep reactions for the preparation of imino- and nitrocyclitols. Adv. Synth. Catal. 2015, 357, 1951-1960. [CrossRef]
Garrabou X, Joglar J, Parella T, Crehuet R, Bujons J, Clapes P. Redesign of the phosphate binding site of L-rhamnulose-1-phosphate aldolase towards a dihydroxyacetone dependent aldolase. Adv Synth Catal. 2011; 353 :89–99.Garrabou, X., Joglar, J., Parella, T., Crehuet, R., ...
Garrabou X, Joglar J, Parella T, Crehuet R, Bujons J, Clapes P. Redesign of the phosphate binding site of L-rhamnulose-1-phosphate aldolase towards a dihydroxyacetone dependent aldolase. Adv Synth Catal. 2011; 353 :89–99.Garrabou X, Joglar J, Parella T, Crehuet R, Bujons J, and...
Karen L. EvansDavid S. FeingoldELSEVIERMethods in EnzymologyChiu, T.H., Evans, K.L., and Feingold, D.S., L-Rhamnulose-1-phosphate aldolase, Meth. Enzymol., 42, 264-269, 1975.Chiu TH, Evans KL, Feingold DS (1975) l -Rhamnulose-1-phosphate aldolase. Methods Enzymol 42:364–369...
The disappearance of L-rhamnulose 1-phosphate added as substrate is measured colorimetrically by the cysteine-sulfuric acid test for the ketohexosesl; or the reaction is followed by determining either dihydroxyacetone phosphate spectrophotometrically with 伪-glycerolphosphate dehydrogenase and reduced ...
Schulz.The structure of l-rhamnulose-1-phosphate aldolase (class II) solved by low-resolution SIR phasing and 20-fold NCS averaging. Acta Crystallographica . 2002Kroemer, M. and Schulz,G.E. (2002). The structure of L-rhamnulose-1-phosphate aldolase (class II) solved by low-resolution ...