Ferrocene-terminated self-assembled monolayer (Fc-SAM) on gold was used as an electron-transfer mediator in the electrochemical assay of L-ascorbic acid 2-phosphate (AAP). The assay is based on the enzymatic action of alkaline phosphatase (ALP), which triggers the release of vitamin C (L-...
Control of Enzymatic Browning in Apple with Ascorbic Acid Derivatives, Polyphenol Oxidase Inhibitors, and Complexing Agents Ascorbic acid-2-phosphate (AAP) and -triphosphate (AATP) showed promise for cut surfaces but were ineffective in juice. Ascorbic acid-6-fatty acid ... GM Sapers,KB Hicks,JG...
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A reaction combination includ- ing leaf extract, 130 mM sodium phosphate buffer, pH 7.4 + 7 mM sodium phosphate buffer, pH 6.8 with 6 mM DTNB, and 5, 5-dithiobis-(2-nitrobenzoic acid) was used to measure the GSH concentration. Prior to measuring the absorbance at 412 nm, the ...
(2′or 3′-azido)-2′,3′-dideoxy-5-fluorocytosine nucleoside (referred to below as “L-(2′ or 3′)-A-5-FddC″) or a pharmaceutically acceptable salt, ester, or prodrug thereof, including a stabilized phosphate, either alone or in combination or alternation with another anti-HIV agent...
Ferrocene-terminated self-assembled monolayer (Fc-SAM) on gold was used as an electron-transfer mediator in the electrochemical assay of L -ascorbic acid 2-phosphate (AAP). The assay is based on the enzymatic action of alkaline phosphatase (ALP), which triggers the release of vitamin C ( L...
Cells: Preosteoblasts of MC3T3-E1 line, subclone 4 (passage 21) (purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany) were thawed and multiplied in culture flasks with a surface of 75 cm2, in culture media α MEM, without ascorbic acid, supplemented with 10% BFS and 1% mixture ...
DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging activity was determined as described by Wu et al. [16]. Ascorbic acid was chosen as the reference standard compound and all tests were done in triplicate. DPPH radical scavenging ability of the test sample was expressed as IC50, the ...
A high-performance liquid chromatography procedure for the determination of ascorbic acid-2-phosphate (AAP), ascorbic acid (AA), and dehydroascorbic acid (DHAA) in raw apple and potato, treated with AAP and AA to prevent browning, was developed. These compounds were extracted with a mixtur...