The L-arabinose molecule (in the C1 pyranose chair conformation) has been fitted to the electron density corresponding to the bound sugar in the 2.4 A resolution Fourier map of the L-arabinose-binding protein. The sugar molecule is buried in the cleft between the two lobes of the bilobate ...
l-Arabinose isomerase and l-ribulokinase, the products of the araA and araB genes, are synthesized at the same rate, demonstrating that the operon is coordinately expressed in vitro. The system is completely dependent upon araC protein which can be supplied either by synthesis in vitro from ...
The sugar-binding site of the L-arabinose-binding protein, an essential component of the high affinity L-arabinose uptake system in Escherchia coli, is located deep in a cleft formed by the asymmetric contributions from both of the two similar domains. The site was unambiguously identified with...
Theoretical conformational energy calculations show that large changes in the width of the binding-site cleft in the L-arabinose-binding protein involve only modest changes in the protein internal energy. Solvation energy changes associated with such variations of the cleft width and with protein-ligand...
The L-arabinose-binding protein of Escherichia coli is a periplasmic component of the bacterial L-arabinose transport system. The three-dimensional structure of the molecule has been determined by x-ray diffraction and shown to have two globular domains and a connecting hinge. Theoretical study of ...
Letter to the Editor: Backbone Resonance Assignment of the L-Arabinose Binding Protein in Complex with D-Galactose Presents a letter to the editor related to backbone resonance assignment of the L-arabinose binding protein in complex with D-galactose. AH Daranas,AP Kalverda,A Chiovitti,... - ...
The L-arabinose-binding protein of Escherichia coli is a periplasmic component of the L-arabinose transport system. Its three-dimensional structure has been determined by x-ray diffraction and shown to have two globular domains and a connecting hinge. These structural features enclose a cleft in wh...
This review covers the physiological aspects of regulation of the arabinose operon in Escherichia coli and the physical and regulatory properties of the operon's controlling gene, araC. It also describes the light switch mechanism as an explanation for many of the protein's properties. Although many...