Enying Zhang, Lei Zhu, Yunyun Hu and Chenwu Xu* Abstract Background: The L-Ala-D/L-Glu epimerases (AEEs), a subgroup of the enolase superfamily, catalyze the epimerization of L-Ala-D/L-Glu and other dipeptides in bacteria and contribute to the metabolism of the murein peptide of pept...
Yang Z, Wang Y, Zhou Y, Gao Q, Zhang E, Zhu L, Hu Y, Xu C: Evolution of land plant genes encoding L-Ala-D/L-Glu epimerases (AEEs) via horizontal gene transfer and positive selection. BMC Plant Biol 2013, 13: 34. Central...
高产L-异亮氨酸谷氨酸棒杆菌的菌种选育及代谢优化[D]. 大连: 大连工业大学, 2016. [12] HAYASHI J, MUTAGUCHI Y, MINEMURA Y, et al. Crystal structure of the novel amino-acid racemase isoleucine 2-epimerase from Lactobacillus buchneri[J]. Acta Crystallographica, 2017, 73(Pt 5): 428. DOI:...
The formation of this disulfide bond also caused a large conformational change in the loop that contains Cys83 (Ala75–Gly84) (Fig. 2c). In the reduced form, the active site exhibits an open confor- mation that is freely accessible to the substrate (Fig. 2d). In contrast, in ...
6E). Four interactions are established between K+ and the main chain carbonyl of Pro-222, Ala-288, Trp-291, and Pro-292, and two interactions with the side chain carboxyl of Glu-221 and Asp-293. Although not directly interacting with K+, residues His-289, Lys-140, Asn-142, and Thr...
The cyclic tetrapeptide of HC-toxin structure comprised of cyclo (D-Pro–L-Ala–D-Ala–L-Aeo), where Aeo represents 2-amino-9,10-epoxi-8-oxodecanoic acid [13]. The HC-toxin is considered as one the microbial secondary metabolite whose ecological function in nature has been studied in ...
hydroxy-D-proline and (2) the irre- versible dehydration of cis-3-hydroxy-L-proline to Δ1-pyrroline-2-carboxylate (Fig. 1c); the latter is homologous with the activities of hydroxyproline betaine 2-epimerase31 and proline betaine racemase32, other members in this protein ...
(xiv) four UDP-glycosyltransferase proteins (UGT89C1, UGT84A1, UGT78D2, UGT84A2), and (xv) one Trifunctional UDP-glucose 4,6-dehydratase/UDP-4-keto-6-deoxy-Dglucose 3,5-epimerase/UDP-4-keto-l-rhamnose-reductase (RHM1) (Fig.3b). Predicted regulatory partners of CHS and CHI proteins...
Gulick, et al., “Evolution of Enzymatic Activities in the Enolase Superfamily: Crystal Structures of the L-Ala-D/L-Glu Epimerases from Escherichia coli and Bacillus subtilis,” Biochemistry 40:15716-15724 (2001). Heim, et al., “Cloning an Escherichia coli Gene Encoding a Protein Remarkabl...
A method is provided for producing an L-amino acid by culturing a microorganism belonging to the Enterobacteriaceae family and having the ability to produce an L-amino acid, in a me