Briefly, lysates were mixed with an equal amount of Laemili sample buffer, separated by 7% SDS-PAGE, and transferred to a nitrocellulose paper. Blots were incubated with a rabbit antibody (1:10,000) directed to the distal C-terminus as previously descri- bed9,10. Bands were detected with...
(b) Sample Western blot (i) of whole cell lysates from stably transfected HEK 293 cells expressing recombinant HA-tagged Kv11.1-1A, Kv11.1-3.1, or Kv11.1-3.1 after 20 hour incubation at 28 °C (Δ T). Lysates were probed with anti-HA and anti-NaK ATPase primary antibodies ...
Although the sample size here is very small because of the rarity of the KCNG4 allele being examined, the finding suggests that an effect of this rare allele is to increase the experimental cuff pressure pain threshold in humans. The experimental cuff pressure pain remains significantly increased...
As such, cell cycle-dependent phosphorylation events can lead to coordinated dissociation of STIM1 from Orai and disruption of this class of PM:ER MCS (37) and formation/stabilization of Kv2.1-containing PM:ER MCS. The protein kinases and phosphatases that regulate cell cycle-dependent clustering...
Although in recent years, there have been significant breakthroughs in the structural biology of ion channels2–4, structures are available only for a handful of channels and the majority of these structures are not for human proteins. The reason for the frustratingly slow progress is that these ...
Molecular biology, virus production and protein expression Full-length human Kv3.1 (isoform A) was cloned from the mammalian gene collection (cDNA clone MGC:129855 IMAGE: 40024733) into LIC-adapted pHTBV C-terminally tagged Strep-II/10-His/GFP vector. Baculoviral DNA from the transformation of ...