验证数据展示 WB analysis of HT-29 using 10885-1-AP HT-29 cells were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.View All Images (5)...
验证数据展示 WB analysis of MCF-7 using 66796-1-Ig MCF-7 cells were subjected to SDS PAGE followed by western blot with 66796-1-Ig (CNKSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.View All Images (4)...
HT-29 cells were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.View All Images (5) Recommended products: Multi-rAb Recombinant Secondary Antibodies New FlexAble 2.0 Antibody Labeling Kits Fe...
Samples were resolved by SDS-PAGE, followed by western blotting. Analysis revealed that KSR1 binds to calmodulin in the presence of Ca2+ (Fig. 1A). Binding was specific as no KSR1 was detected in samples incubated with GST-Sepharose. Ca2+ regulates the interaction of calmodulin with numerous...
简单介绍 CNKSR1Antibody CNKSR1 Antibody的详细介绍 CNKSR1Antibodyis a Rabbit Polyclonal against CNKSR1. TargetCNKSR1 ReactivityHuman, Mouse, Rat HostRabbit ClonalityPolyclonal Tested ApplicationsELISA, WB, IHC Purity> 95% (SDS-PAGE) PurificationImmunogen affinity purified. ...
Phosphorylated KSR1 proteins were separated by SDS-PAGE, transferred to nitrocellulose membranes, and quantitated using a phosphoimager. The activities of Chk1 and Chk2 were confirmed using the CHKtide substrate, and the activity of Akt was demonstrated using the Crosstide substrate (data not ...
In brief, lysates were size- separated on a 10% SDS-PAGE gel followed by tank blot transfer to Polyscreen polyvinyl difluoride membranes (Perkin Elmer). Proteins of interest were detected with the following primary antibodies: anti-RAF1 (#9422), anti-MEK1/2 (#9122), Ras (G12V Mutant ...
After 24 h, proteins were extracted, mixed 1 : 1, separated on SDS–PAGE, trypsin- digested, fractionated and analysed by LC-MS/MS using Max- Quant software (Cox and Mann 2008; Figure 1A). We identified a total of 2504 proteins out of which 2032 were quantified (false discovery rate ...
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