Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable ...
Sphinganine is a key precursor for the synthesis of ceramide during sphingolipid biosynthesis in eukaryotes. In the sphinganine biosynthetic pathway, serine palmitoyltransferase (SPT) and 3-ketosphinganine reductase (3KSR) catalyze the condensation of serine and palmitoyl-CoA to form 3-ketosphinganine, ...
containing no serum; and based on the volume of freezing medium free of serum, the content of free culture medium is serum nurse is 5% -15% (v / v) DMSO content is byb is 8% - 20% (v / v), and the content is cyc KSR is 70% -85% (v / v), while a + b + c <= ...