The bacteria of this genus are widely distributed and can be detected in the soil, sewage, spoilage animals and plants, and the intestines of humans and animals in nature. In recent years, the widespread use of antibacterial drugs in the clinic, improper drinking of the contamination of water...
目的分析1株亚胺培南抗性阿斯肠杆菌(Enterobacter asburiae)的耐药机制及其遗传特征.方法Vitek-2 Compact系统初步鉴定菌株并测定抗生素最小抑菌浓度,对16s rRNA基因测序以确定菌株;PCR法扩增β-内酰胺类,喹诺酮类和氨基糖苷类等17种耐药基因并测序确认;以CaCl2诱导的化学法转化质粒;构建伴生质粒DNA文库并测序,以Glimmer 3....
Carbapenems are β-lactam antibiotics regarded as the last line for the treatment of infections caused by multidrug-resistant gram-negative bacteria, including K. pneumoniae [3]. Nonetheless, this bacterium has acquired resistance to this class of antibiotics (Carbapenem-resistant K. pneumoniae, CR-...
Conclusions KPC-2and IMP-4 genes are the major carbapenemase genes in Klebsiella pneumoniae isolated in Wuhan.Transmission of drug resistance is mainly through vertical transmission of type C resistant clone and horizontal transmission of Intl on bacteria chromosome.虞涛...
Timely microbiological diagnosis and optimizing antibiotic dosing regimens are essential to prevent worse outcomes. Further studies and well-controlled clinical trials are needed to define the optimal treatment of infections by KPC-Kp and, more generally, carbapenem-resistant bacteria....
1.2.4 基因环境分析 用blaKPC⁃2基因引物对目的细菌基因组DNA进行基因扩增。扩增产物纯化后送博尚生物公司测序,测序结果经http://blast.ncbi.nlm.nih.gov/Blast比对,获得KPC的基因环境。 2.1 KPC阳性菌株筛选及药敏试验 通过PCR筛选及测序确认,286株碳青霉烯类抗生素敏感性下降的革兰阴性菌中有40株(占14%)携带有...
The emergence of Klebsiella pneumoniae carbapenemases (KPCs) producing bacteria has become a significant global public health challenge while the optimal treatment remains undefined. We performed a systematic review of published studies and reports of treatment outcomes of KPC infections using MEDLINE (2001...
(CRKP).Understanding the resistance mechanism and infection status of CRKP antibacterial to carbopenems.Provide the basis for the clinical anti infection treatment and hospital infection control.Methods The application of BD phoenixTM-100 automatic bacteria identification and drug sensitivity analysis system...
Tothetetracyclines(i.edoxycycline),itisimportanttonotethatMIC90valuesareoftenatorneartheCLSIsusceptibilitybreakpoint(4mg/L).Clinicallyachievabledrugconcentrationsatthesiteofinfectionshouldbe takenintoaccountbeforeusingthisclassofagents.1.BratuS,MootyN,NichaniSetal.AntimicrobAgentsChemother2005;49:3018–20.2...
GRAM-negative bacteriaTERTIARY careKLEBSIELLA infectionsBackground: Klebsiella pneumoniae infections have become a major cause of hospital acquired infection worldwide with the increased rate of acquisition of resistance to antibiotics. Carbapenem resistance mainly among Gram negative is an ...