Then, 442 SNP markers were successfully converted into kompetitive allele-specific PCR (KASP) markers, accounting for 88.4% of all 500 SNP markers. The cabbage inbred lines included three ecotypes: spring cabbage, autumn cabbage and winter cabbage. Based on the KASP genotyping data, the genetic...
Kompetitive Allele Specific PCR MAF, minor allele frequency; MEGA: Molecular evolutionary genetics analysis; NGOs: Non-governmental organizations; OPVs: Open-pollinated varieties; QC: Quality control analysis; SNP: Single nucleotide polymorphism; SSA: Sub Saharan Africa; UPGMA: Unweighted pair group ...
REVIEWSingle nucleotide polymorphism genotyping usingKompetitive Allele Specific PCR (KASP): overviewof the technology and its application in crop improvementKassa Semagn • Raman Babu • Sarah Hearne •Michael OlsenReceived: 5 April 2013/Accepted: 1 July 2013/Published online: 10 July 2013?
Genotyping-by-sequencing (GBS) based single nucleotide polymorphisms (SNP) enabled Kompetitive allele specific PCR (KASP) marker development in mutant Rubus genotypesdoi:10.1016/j.ejbt.2018.08.001Ryu, JaihyunkKim, Woon JiIm, JuhyunKim, Sang Hun...
Comparison of Kompetitive Allele Specific PCR (KASP) and genotyping by sequencing (GBS) for quality control analysis in maize. Quality control (QC) analysis is an important component in maize breeding and seed systems. Genotyping by next-generation sequencing (GBS) is an emerging m... Berhanu,...
These reliable SNPs were analyzed using the Kompetitive Allele Specific PCR (KASP) assay to discriminate among persimmon genotypes. GBS generated a total of 447,495,724 trimmed reads, of which 89.7% were raw reads. After demultiplexing and sequence quality trimming, 108,876,644 clean reads were...
The objectives of our study were 1) to evaluate the level of genetic purity and identity among two to nine seed sources of 16 inbred lines using 191 Kompetitive Allele Specific PCR (KASP) and 257,268 GBS markers, and 2) compare the correlation between the KASP-based low and the GBS-...
Ertiro BT, Ogugo V, Worku M, Das B, Olsen M, Labuschagne M, Semagn K (2015) Comparison of Kompetitive Allele Specific PCR (KASP) and genotyping by sequencing (GBS) for quality control analysis in maize. BMC Genomics 16:908 Central...
Modifications of Kompetitive Allele-Specific PCR (KASP) Genotyping for Detection of Rare Alleles.KASP is commonly used to genotype bi-allelic SNPs and In/Dels, and the standard protocol works well when both alleles are nearly equally prevalent in the DNA template. To detect rare alleles in ...
Ertiro BT, Ogugo V, Worku M, Das B, Olsen M, Labuschagne M, Semagn K (2015) Comparison of Kompetitive Allele Specific PCR (KASP) and genotyping by sequencing (GBS) for quality control analysis in maize. BMC Genomics 16:908 Central...