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Several of the following behavioral assays have been conducted in more than one batch of animals and in both male and female mice. Unless otherwise stated, the data shown in the primary figures are from the batch of males with most animals, and the additional data sets are shown in theSuppl...
Conditional knockout using Cre/lox is essential for functional analysis of genes. CRISPR/Cas in combination with two sets of guide RNAs and a single-stranded oligonucleotide enables simultaneous insertion of two lox sequences. However, this method induces double-strand breaks at two sites on the ...
TMTPost -- China’s top electric vehicle manufacturer BYD Co. Ltd. set a relatively conservative growth target for the year 2024 in face of fierce competition in the EV market. Credit:Transport Buearu of Shenzhen Municipality BYD aims to increase its sale by more than 20% on the top of ...
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We performed MeSH analysis39 to determine if genes with altered Pol II signals in Top3β-KO mice are associated with any gene sets important for psychiatric disorders. We identified dementia and schizophrenia as Top10-ranked negative MeSH terms among 1661 total terms (Fig. 7a, b; Supplementary...
However, the additional richness of the sequencing data set, namely the read counts, allows for the connection of sets of coordinates and deduction of the location of non-unique mutants (Methods). A priori, we expect that the frequency of a given DNA amplification event should not vary ...
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