Inc. (New York) were bred to yield WT, HT, and KO mice that were weaned at week-3, genotyped using primers specific for theGabrb2andNeogenes (Supplementary Methods) and housed in groups of four to six with water and food ad lib, with a 12-h light cycle from 08:00 to 20:00. A...
Writing on the Web is a technology we are just starting to learn to use. While writing for the Web can be seen as formally and functionally close to writing for print, such a perspective deprives it of the opportunities hypermedia environments and its texts open for us to connect, understan...
Mutations in the LRRK2 gene are the most common cause of genetic Parkinson’s disease. Although the mechanisms behind the pathogenic effects of LRRK2 mutations are still not clear, data emerging from in vitro and in vivo models suggests roles in regulati
1994). To this end, we created a targeting vector that introduced a gene cassette consisting of the neomycin resistance (neo) and herpes simplex virus thymidine kinase (HSV-Tk) genes flanked by loxP sites upstream of exon 4 of the IR gene and a single loxP site 80 bp downstream of exon...
For instance, the Mb KO models (Garry et al. 1998; Gödecke et al. 1999) were made by replacing exon 2 with a neo selection cassette (Supp. Figure 1), which poten- tially interferes with expression of other genes (Pham et al. 1996; Scacheri et al. 2001; Meier ...
The knockout of Immp2l activity and the treatment with MitoQ each independently reduced the ratio of open-arm entries (genotype main effect: F(1,56) = 8.262, p = 0.006; treatment main effect: F(1,56) = 7.850, p = 0.007; Figure 3E), which indicates increased avoidance of the ...
000 or goat anti-rabbit at 1:2500 HRP-linked antibody (Cell Signaling) in blocking solution. Following three TBST washes, bands were visualized using chemiluminescent Clarity Max Western ECL Substrate (Bio-Rad). The Western blot images were analyzed using an open-source software Fiji ImageJ (...