However, the bsAb byproducts and impurities, including mispaired homodimers, half-antibodies, light chain mispairings, antibody fragments and high levels of high molecular weight (HMW) species, all pose unique challenges to their downstream processing. Here, using two knob-into-hole (KiH) ...
在knob-into-hole技术的基础上,单特异性抗体分子上加了Fc-dummy chains,避免半抗体样分子(half-antibody like molecules)聚集,形成二聚体。在input A和Input B加入的两个半抗体样分子相遇,则发生Format Chain交换,两个Fc-dummy chains带有不同电荷,及Konb和hole,自组装成二聚体(Dummy dimmer)在纯化时,可以被去掉。
在knob-into-hole技术的基础上,单特异性抗体分子上加了Fc-dummy chains,避免半抗体样分子(half-antibody like molecules)聚集,形成二聚体。在input A和Input B加入的两个半抗体样分子相遇,则发生Format Chain交换,两个Fc-dummy chains带有不同电荷,及Konb...
Structural and Functional Characterization of a Hole-Hole Homodimer Variant in a "Knob-Into-Hole" Bispecific Antibody Compared with the hole–hole homodimer under different storage conditions, the bispecific heterodimer, guided by the knob-into-hole assembly, proved to be a stable conformation with ...
Non-affinity platform for processing knob-into-hole bispecific antibody Bispecific antibodies hold significant potential as next-generation biotherapeutics owing to their ability to simultaneously bind to two targets. However, ... Xiaoyang Wang,Min Li,Mengting Li,... - Bioresources and Bioprocessing 被...
6、'Knobs-into-holes' engineering of antibody CH3 domains for heavy chainheterodimerization 7、A new approach for generating bispecific antibodies based on acommon light chain format and the stable architecture of human immunoglobulin G1 声明:本文章以宣传知识为目的,因此不构成投资,病人用药等建议。如果...
bispecific antibodyknob-into-holechromatography purificationlow pH washhole-hole homodimeraggregation propensityBispecific antibodies (bsAbs) are therapeutically promising due to their ability to bind to two different antigens. However, the bsAb byproducts and impuritiesZhang, Wei...
bispecific antibodyassemblypurificationknobs into holeshigh‐throughput screeningProduction of knob and hole dual light chain bispecific antibodies poses several unique challenges for development of a feasible industrial scale manufacturing process. We developed an efficient process for the assembly and ...
Bispecific antibodyKnob-into-holeFlow-through polishingHost cell proteinsHigh molecular weight impuritiesLow molecular weight impuritiesHigher aggregation propensityBispecific antibodies (bsAbs), though possessing great therapeutic potential, are extremely challenging to obtain at high purity within a limited ...
Knob-into-Holeprotein crystallographyAn increasing number of bispecific therapeutic antibodies are progressing through clinical development. The Knob-into-Hole (KiH) technology uses complementary mutations in the CH3 region of the antibody Fc fragment to achieve heavy chain heterodimerization. Here we ...