从米氏常数_Km_的测定谈药学生物化学实验教学
从米氏常数(Km)的测定谈药学生物化学实验教学 马中良 1 李艳利 2 鲍真真 1 王 1 1 中国药科大学 (南京 210009); 2 南京医科大学(南京 210029) 摘要 在生物化学试验中,酶的米氏常数的测定实验是经典的实验。通过Km测定这一实验的改进,指导学生怎 样认识和把握理论知识,并将之应用科学研究中。在生物化学实验教...
点个赞。但IC50和EC50可以跟小白们解释得更加简明一些。IC50的I，重在Inhibition，只考察对靶点的效应。EC50的E，重在Effect，看的是总体生物学表型。举例：抗癌药EGFR激酶抑制剂的IC50，是指EGFR的磷酸化被抑制一半的剂量，而EC50是指癌细胞的活力被抑制一半的剂量。
American Society for Biochemistry and Molecular BiologyJournal of Biological ChemistryMcLaughlin MM,Cieslinski LB,Burman M,Torphy TJ,Livi GP.A low-Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain:cloning and expression of cDNA, biochemical characterization of recombinant protein, ...
(Biochemistry) a substance that stimulates the production of antibodies [C20: from anti(body) + -gen] ˌantiˈgenicadj ˌantiˈgenicallyadv Collins English Dictionary – Complete and Unabridged, 12th Edition 2014 © HarperCollins Publishers 1991, 1994, 1998, 2000, 2003, 2006, 2007, 200...
Webb,Andrej Lupták - 《Biochemistry》 被引量: 0发表: 2018年 Improvement of efficiency of the Ce(IV)-induced DNA scission — Relationship between the kinetic parameters (kcat and Km) and the DNA structure- Improvement of efficiency of the Ce(IV)-induced DNA scission — Relationship between...
- 《Biochemistry》 被引量: 131发表: 1974年 Isolation and Purification of Tyrosine Hydroxylase from Callus Cultures of Portulaca grandiflora. The Km values for tyrosine and 6-methyl-5,6,7,8-tetrahydropterin were 0.5 mM and 0.15 mM, respectively. This enzyme was activated by Fe2+ and Mn2.....
358；David L Nelson, Michael M Cox. Lehninger Principle of Biochemistry[M], 7th edition.
- 《Molecular & Cellular Biochemistry》 被引量: 83发表: 2002年 ATP and phosphate reciprocally affect subunit association of human recombinant High Km 5′-nucleotidase. Presents a study which generated several structural modifications of the inosine monophosphate (IMP)-specific, high Km 5 ′-...
[11] Cárdenas, M. L. (2004). “Comparative biochemistry of glucokinase”in Glucokinaseand glycemic disease: From basics to novel therapeutics.eds. F. M. Matschinsky and M. A. Magnuson (Basel: Karger), 31–41. [12] Agius, L. (2016). Hormonal and metabolic regulation of hepaticglucokina...