Agarwal R,McBean M,Hewitt C,et al.KIT D816V mutation detection:a comparative study using peripheral blood(PB),bone marrows aspirate(BMA) and bone marrow trephine(BMT) samples for detection of KIT mutations in patients with systemic mastocytosis[J].Leuk Lymphoma,2014.[Epub ahead of print]....
The c-kit D816V activating mutation is found in >80% of cases of systemic mastocytosis (SM) and represents a potential drug target. Furthermore, because D816V is one of the diagnostic criteria for SM, it is clinically relevant to determine whether the mutation is present. Traditional techni...
The most common mutation of c-Kit in these disorders is a substitution of the aspartic acid residue in position 816 to a valine (D816V), leading to constitutive activation of the receptor. In this study, we aimed to investigate the role of Src family kinases in c-Kit/D816V signaling. ...
Mutation D816V alters the internal structure and dynamics of c-KIT receptor cytoplasmic region: implica- tions for dimerization and activation mechanisms. PLoS Comput Biol 2011;7: e1002068.Laine E, Chauvot de Beauchene I, Perahia D, Auclair C, Tchertanov L. 2011. Mutation D816V alters ...
[translate] aPCR方法的建立,根据等位基因特异性PCR法(AS-PCR)的原理,设计一对特异引物,以含D816V突变的 pEGFP- C1- c-kit重组质粒为模板,通过普通PCR优化退火温度后再优化荧光定量PCR条件。 正在翻译,请等待... [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 ...
(D) Ba/F3 cells expressing KIT-D816V were washed three times and then serum- and cytokine-starved for 4 hours. Cells were lysed and subjected to immunoprecipitation with an anti-STAT3 antibody. Statistical analysis was performed by quantifying Western blots from three independent experiments. ...
The c-Kit/D816V mutation eliminates the differences in signal transduction and biological responses between two isoforms of c-Kit. Cell Signal. 2009;21:413-8 44. Kazi JU, Vaapil M, Agarwal S, Bracco E, Pahlman S, Ronnstrand L. The tyrosine kinase CSK associates with FLT3 and c-...
Mutations in the juxtamembrane and tyrosine kinase domains of the KIT receptor havebeen implicated in several cancers and are known to promote tumorigenesis. However,the pathophysiological manifestations of mutations in the extracellular domainremain unk
PKC412是一 1eukemieMastCellLeukemiawiht AbnormalImmunophenotypenadC— 种有用的治疗试剂在46%核心结合因子白血病的C—Kit kitMutationD816VLeukemia& Lymphoma.2004;45:2295—2302 12TobiasM Kohi,Susanne~hnittger,JoachimW Ellwart,eta1.KITexon 中,酪蛋白激酶结构域(1KI)),近膜结构域,外显子8及内 8...
Mastocytosis is typically associated with the D816V mutation in the gene encoding KIT, which results in the constitutive activation of the receptor. Mast cells can be generated from those patients even without cytokine stimulation.46 Our findings show that IL-3 and IL-6 are sufficient for mast...