Agarwal R,McBean M,Hewitt C,et al.KIT D816V mutation detection:a comparative study using peripheral blood(PB),bone marrows aspirate(BMA) and bone marrow trephine(BMT) samples for detection of KIT mutations in patients with systemic mastocytosis[J].Leuk Lymphoma,2014.[Epub ahead of print]....
Here, an allele-specific assay to detect the D816V mutation in DNA from archived formalin-fixed paraffin-embedded tissues is described. A two-tube PCR format was employed to amplify c-kit exon 17 as a control and an allele-specific reaction to selectively amplify the D816V mutant allele ...
rate after one course salvage chemotherapy and the relationship between kit mutation and cr 2 rate was analyzed. results 68 cases were enrolled in this study, and 30 cases (44.1%) achieved cr 2 . all patients received kit mutation detection, and kit d816 mutation was identified in 26 cases...
结论:变性高效液相色谱技术是一种高效、可靠的突变检测方法,c-kit基因突变检测对肥大细胞病临床治疗有指导意义关键字:变性高效液相色谱;c-kit基因突变;D816V;L862L;肥大细胞病ApplicationofDenaturingHighPerformanceLiquidChromatographytoMutationDetectionofthec-kitGeneinMastocytosisAbstractTheaimofstudywastodetectmutation...
The antitumor activity of homoharringtonine against human mast cells harboring the KIT D816V mutation. Gain-of-function mutations of the receptor tyrosine kinase KIT play a critical role in the of (SM) and . The various juxtamembrane type of KIT mutations, i... Y Jin,Z Lu,K Cao,... -...
Mutation D816V alters the internal structure and dynamics of c-KIT receptor cytoplasmic region: implica- tions for dimerization and activation mechanisms. PLoS Comput Biol 2011;7: e1002068.Laine E, Chauvot de Beauchene I, Perahia D, Auclair C, Tchertanov L. 2011. Mutation D816V alters ...
[translate] aPCR方法的建立,根据等位基因特异性PCR法(AS-PCR)的原理,设计一对特异引物,以含D816V突变的 pEGFP- C1- c-kit重组质粒为模板,通过普通PCR优化退火温度后再优化荧光定量PCR条件。 正在翻译,请等待... [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 ...
Human c-KIT D816V Mutation Detection Kit CBP00298K Human c-KIT V560D Mutation Detection Kit CBP00302K Human EGFR A767_V769dupASV Mutation Detection Kit CBP00162K Human EGFR C797S Mutation Detection Kit CBP00156K Human EGFR D770_N771insSVD Mutation Detection Kit CBP00164K ...
(D) Ba/F3 cells expressing KIT-D816V were washed three times and then serum- and cytokine-starved for 4 hours. Cells were lysed and subjected to immunoprecipitation with an anti-STAT3 antibody. Statistical analysis was performed by quantifying Western blots from three independent experiments. ...
The c-Kit/D816V mutation eliminates the differences in signal transduction and biological responses between two isoforms of c-Kit. Cell Signal. 2009;21:413-8 44. Kazi JU, Vaapil M, Agarwal S, Bracco E, Pahlman S, Ronnstrand L. The tyrosine kinase CSK associates with FLT3 and c-...