TEREFE N S,VAN-LOEY A,HENDRICKX M.Modelling the kinet-ics of enzyme-catalysed reactions in frozen systems:the alkaline phos-phatase catalysed hydrolysis of di-sodium-p-nitrophenyl phosphate. Innovative Food Sci Emerging Technol . 2004N.S. Terefe, A. Van Loey, M. Hendrickx, Innov Food Sci ...
IV. Kinetics of the overall reaction catalysed by preparations of the purified enzyme. 1. 1. The kinetics of the reaction catalysed by succinate dehydrogenase (EC 1.3.99.1) have been studied. Double reciprocal plots (inverse of velocity against inverse of succinate concentration) at various ...
If the change in temperature does not (a) inactivate the enzyme, (b) alter the affinity of the enzyme for the substrate, an activator or an inhibitor or (c) alter the pH function of the reaction components, the velocity of enzyme catalysed reactions increases with increasing temperature. The...
has been developed using a schematic method devised by King and Altman [E.L. King, C. Altman, A schematic method of deriving the rate laws for enzyme catalysed reactions, J. Phys. Chem. 60 (1956) 1375–1378] for deriving the rate equations for a complex enzyme-catalysed reaction. PAC...
Morrison JF (1969) Kinetics of reversible inhibition of enzyme-catalysed reactions by tight-binding inhibitors. Biochim Biophys Acta 185:269–286. https://doi.org/10.1016/0005-2744(69)90420-3 Article CAS PubMed Google Scholar Mure M et al (2005) Role of the interactions between the active...
Analysis of immobilised multi‐enzyme reaction systems with michaelis‐menten kinetics The consecutive sequence of reactions S → P 1 → P 2 taking place inside a permeable spherical particle is analysed for the case in which the enzyme‐catalysed steps in the reaction follow Michaelis‐Menten type...
Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions. ... Studying an enzyme's kinetics in this way can revealthe catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or a modifier (inhibitor or activator) mi...
reactions produce or consume heat; ITC can be applied equally well to virtually any enzyme16, and does not require the development of a customized assay based on fluorogenic or colorigenic substrates, or the post-reaction separation of products and substrates by chromatography or electrophoresis16,...
Michaelis-Menten kinetics: explicit dependence of substrate concentration on reaction timeENZYME kineticsBIOCHEMICAL engineeringThe nonlinear dependence of the rate expressions associated with enzyme-catalysed reactions on the concentration of substrate implies that the corresponding integrated form of the ...
Experimental designs for estimating the parameters of the Michaelis-Menten equation from progress curves of enzyme-catalyzed reactions When the progress curve for an enzyme catalysed reaction follows the integrated Michaelis-Menten equation, the maximum velocity and Michaelis constant can ... RG Duggleby...