Universal Kinase Activity Kit Assay Principle 你在这里 Home»Resources»Technical Information» Universal Kinase Activity Kit Assay Principle An reaction mixture containing CD39L2 and the kinase is added to each well of a 96-well microplate....
Assay principle The principle of the LanthaScreen Eu Kinase Binding Assay is shown in Figure 1. Binding of an Alexa Fluor™ conjugate or “tracer” to a kinase is detected by addition of a Eu-labeled anti-...
Assay principle The principle of the LanthaScreen Eu Kinase Binding Assay is shown in Figure 1. Binding of an Alexa Fluor™ conjugate or “tracer” to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer and antibody to a kinase results in a high ...
Assay principle The principle of the LanthaScreen Eu Kinase Binding Assay is shown in Figure 1. Binding of an Alexa Fluor™ conjugate or “tracer” to a kinase is detected by addition of a Eu-labeled anti...
View Universal Kinase Activity Assay Principle TheUniversal Kinase Activity Kit(Catalog # EA004) provides a simple, non-radioactive, high-throughput compatible format for assaying the enzyme activity of kinasesin vitro. The majority of kinases use ATP as the phosphate donor. They transfer the termin...
The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP. Figure 1. Principle of the ADP-Glo™ Kinase Assay. The ATP remaining after completion of the kinase reaction is depleted prior to an...
Enzymatic Assay of ACETATE KINASE1 (EC 2.7.2.1) PRINCIPLE: Acetate + ATP Acetate Kinase> Acetyl Phosphate + ADP ADP + PEP Pyruvate Kinase> ATP + Pyruvate Pyruvate + ?-NADH Lactic Dehydrogenase> Lactate + ?-NAD Abbreviations used: ATP = Adenosine 5'-Triphosphate ADP = Adenosine 5'-Diphosphat...
KinaseGlo is a luminescent kinase assay system that utilizes recombinant kinases and firefly luciferase to detect kinase activity inreal-time. The assay is based on the principle that when the recombinant kinase phosphorylates a peptide substrate, the released phosphate can be transferred to ATP, resu...
UniversalKinaseActivityKit CatalogNumber:EA004 Theprotocolpresentedisfora96-wellformat.Materials providedaresufficientfortwo96-wellmicroplatesor equivalent.Thispackageinsertmustbereadinitsentirety beforeusingthisproduct. ASSAYPRINCIPLE TheUniversalKinaseActivityKitprovidesaconvenient, non-radioactivehigh-throughputcompatib...
METHODS: Recent literatures on these tyrosine kinase assay technologies are reviewed, each assay principle, advantages and drawbacks, as well as their potential utilities in tyrosine kinase drug discovery are discussed. RESULTS/CONCLUSION: There is no perfect assay yet; the choice of assay technology ...