析(上海康成生物有限公司)。使用GenePix4000B芯片扫描仪扫描芯片的荧光 强度。采用LuxScan3.O图像分析软件(CaPitalBio公司)对芯片图像进行分析处 理,荧光染料cy3的信号用绿色表示,cy5的信号用红色表示。整合两张芯片荧光 强度的比值,IPRatio值,以整合后的Ratio值>12.0或40.5为标准确定差异表 ...
实用医学杂志2016年第32卷第16期 乳腺癌已成为全球女性发病率以及病死率最 高的恶性肿瘤 [1] ,而中国女性乳腺癌的发病率在亚 洲处于较高水平 [2] ,且有逐年上升及年轻化的趋 势。组织芯片是一种特殊的生物芯片技术,是将许 多不同个体组织标本以规则阵列方式排布于 载玻片上,进行同 指标的原位组织学研究。
Gene Info — KIF2A Interactomes Diseases Related Products PAB31125 KIF2A polyclonal antibody PAB31050 KIF2A polyclonal antibody PAB12407 KIF2A polyclonal antibody H00003796-M01 KIF2 monoclonal antibody (M01), clone 2D4 H00003796-K KIF2A rabbit monoclonal antibody...
Gene Symbol KIF2A Gene ID (NCBI) 3796 Conjugate Unconjugated Form Liquid Purification Method Protein G Magarose purification UNIPROT ID O00139 Storage Buffer PBS Only Storage Conditions Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°CProt...
Purity> 95% PurificationProtein G Purified. IsotypeIgG ConjugationBiotin StorageAliquot and store at -20 °C or -80 °C. Avoid repeated freeze/thaw cycles. Swiss ProtO00139 GeneID3796 OMIM62591 NCBI AccessionNP_001091981.1 NM_001098511.2 [O00139-4] NP_001230881.1 NM_001243952.1 [O00139-5] NP...
pCMV-SPORT6-KIF2A(42-2121bp)Addgene NGS results GTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGC GGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAAC TATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAA GGAGAAAATACCGCATCAGGCGCCATTCGCC...
Small interfering RNAs (siRNAs) of Kif2a (Gene Pharma) were microinjected into the cytoplasm to knockdown Kif2a. The subsequent sequences of Kif2a siRNAs were used at 20 μM each, Kif2a siRNA-1: 5′- GGCCACUAGUGGAAACAAUTT-3′; Kif2a siRNA–2: 5′-GGAUGUUGAUGCUACA...
背景资料:The protein encoded by this gene is a plus end-directed motor required for normal mitotic progression. The encoded protein is required for normal spindle activity during mitosis and is necessary for normal brain development. Several transcript variants encoding different isoforms have been foun...
In this study, we knocked down the Es-Kif2a gene by injecting dsRNA into E. sinensis and inhibited Es-Plk1 gene expression by injecting PLK1 inhibitor BI6727 into E. sinensis. Then, the cell proliferation level, apoptosis level, and PI3K/AKT signaling expression level were detected. Our ...
The aim of the present study was to investigate the expression of Ki-67 in human laryngeal squamous carcinoma and the effect of Ki-67 gene silencing by small interfering (si)RNA on the proliferation of human laryngocarcinoma HEp2 cells. Immunohistochemistry and reverse transcription-quantitative ...