“Cuylen, Sara et al. “Ki-67 acts as a biological surfactant to disperse mitotic chromosomes.” Nature vol. 535,7611 (2016): 308-12. doi:10.1038/nature18610”. [4]“Bruno, S, and Z Darzynkiewicz. “Cell cycle...
Ki67是细胞增殖相关的核抗原, Ki67表达高低与细胞周期中的有丝分裂相关, 与肿瘤发展密切相关。Ki67在肿瘤组织中呈周期性高表达, 在正常组织中低表达, 是乳腺癌分子分型及预后重要指标, 目前有研究表明其在乳腺癌治疗中可用于评估治疗疗效及预后, Ki67是现有新辅助...
关键词:细胞周期;流式细胞术;Ki67;4′,6‐二脒基‐2‐苯基吲哚;碘化丙啶ApplicationofKi67andnuclearacidfluorescentdyeco‐staininginanalysisofcellcycleSUNShuhuiDepartmentofMedicalMicrobiologyandParasitology,KeyLaboratoryofMedicalMolecularVirologyofMinistriesofEducationandHealth,SchoolofBasicMedicalSciences,Fudan...
Ki67 is an intrinsic marker of ongoing cell proliferation and is present during all active phases ofthe cell cycle (G1-M). This antibody is polyclonal and was raised in rabbit to the human peptidesequence – TPKEKAQALEDLAGFKELFQT – that was coupled to thyroglobulin byglutaraldehyde before be...
背景介绍Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms...
This study was conducted to generate a genetic based reporter to label cells that transitioned from the G0 to G1/S phases of the cell cycle, hypothesizing that the proximal promoter of the Ki67 (Ki67p) gene, a commonly used cytology marker induced during this transition, would contain the ...
[J].CellCycle, cytologyinthetriageofASCUSandLSILpapanicolaoucytology 2004,3(5)611615. resultsfromtheEuropeanequivocalormildlyabnormalPapanico [17]McLaughlinDrubinME,ParkD,MungerK.Tumorsuppressor laoucytologystudy[J].CancerCytopathol,2011,119(3)158 p16INK4Aisnecessaryforsurvivalofcervicalcarcinomacell...
4 Cell cycle of umbilical cord MSCs cells of conflu‐ ence 70%—90% 3.6 对比 CFSE 法与 Ki67 法检测 MSCs 的免疫 调控 流式细胞仪检测各组细胞 ,通过 CFSE 荧光强 度的变化观察淋巴细胞增殖情况 ,CFSE 荧光强度 Fig. 5 图 5 5 批脐带 MSCs 的生长曲线 Growth curve of of five batches of ...
【Keywords】Ki67 labeling; cancer cell; growth fraction; cell cycleKi67 labeling should enter the textbook of pathologyQIN Jun-Hui 1,2 , LIANG Yuan 1,2 , WANG Rui-An 1,21 Department of Pathology, Xijing Hospital, 2 Department of Pathology and Pathophysiology, School of Basic Medicine, Fourth...
1、Ki-67，在很多肿瘤病理中做，它是判断肿瘤细胞增殖情况的一个指标，越高表示正在肿瘤细胞增殖多，恶性程度越高。2、P16，是一个抑癌基因，直接作用于细胞周期、抑制细胞分裂的基因，细胞内P16蛋白减少，会引起细胞周期调节紊乱，使细胞无限制增生，甚至癌变。3、P16、Ki-67联合应用可作为宫颈良性病变...