Cas9-Cell line Knock-In service(Cas9-CKI)基因定点敲入细胞系是Cas9X技术团队针对实验室常用的各种细胞系,研究并确立针对不同的细胞的外源基因或者片段高效敲入(Knock-in, KI)的技术操作规程,主要目的就是:解决KI效率低下和KI片段长度限制的问题。 目前使用Cas9的进行基因KI研究方法,主要应用在目的基因后面加上标签...
10月30日,中科院生物化学与细胞生物学研究所周斌研究组在Cell Reports在线发表了最新成果“Apj+ vessels drive tumor growth and represent a tractable therapeutic target”。该研究利用Apj-CreER和Apj-DTRGFP-Luc基因敲入小鼠模型,分别建立皮下肿瘤移植模型、原位肿瘤移植模型、基因缺陷原位肿瘤模型和化合物诱导肿瘤模型,...
7、Olszewski AJ, Butera JN, Reagan JL, Castillo JJ. Outcomes of bendamustine- or cyclophosphamide-based first-line chemotherapy in older patients with indolent B-cell lymphoma. Am J Hematol. 2020 Apr;95(4):354-361. 审校:Moly 排版:Moly ...
Effects of H3K27 methylation inhibitor EPZ005687 on apoptosis, proliferation and cell cycle of U937 cells and normal CD34 positive cells. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2014 Dec;22(6):1561-6 规格 5mg 10mg EPZ005687是一种有效的,选择性EZH2抑制剂。 发表在 小分子化合物 | 标签为 ...
基因Mc3R、基因Mc4R 双基因敲除大鼠 TALEN基因敲除小鼠 斑马鱼等模式动物基因敲除模型 Cas9-Cell Line Gene Knock-Out service(CGKO)基因敲除细胞系服务 细胞基因敲除服务 基因敲除细胞株 请按照相关文献和相关安全规定进行操作,避免误用或滥用。如有侵权,可联系我们删除...
【文献解读】KI小鼠模型揭示肿瘤血管新生的新靶标 10月30日,中科院生物化学与细胞生物学研究所周斌研究组在Cell Reports在线发表了最新成果"Apj+ vessels drive tumor growth and represent a tractable therapeutic target". 10月30日,中科院生物化学与细胞生物学研究所周斌研究组在Cell Reports在线发表了最新成果"Apj+...
[4]. Eyre TA, Bishton MJ, McCulloch R, et al. Diagnosis and management of mantle cell lymphoma: A British Society for Haematology Guideline. Br J Haematol. 2024;204(1):108-126. doi:10.1111/bjh.19131. [...
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The Ki-67 protein can be used as a marker for cell proliferation assay because it is expressed throughout the active cell cycle (G1, S, G2, and M phases).
Abstract: Aim To construct the recombinant co-expression vector carrying antisense RNA to Ki-67 and Bcl-2 genes and which will be used to resist tumers. Methods RT-PCR was used to amplify the orF of Ki-67 and Bcl-2 cDNA from total RNA of Gastric carcinoma cell line SGC-7901. The...