PCR duplicates are defined as sequencing reads resulting from two or more PCR amplicons of the same DNA molecule. Although bioinformatic tools are available to identify and remove PCR duplicates during data analysis [18], minimizing ...
NK cells are a key component of innate immunity and, to a large extent, promote the antitumor immune response [79, 80]. The function of NK cells, such as immune surveillance, is also affected by age-related changes. The number of mature NK cells in all lymphoid organs and the expression...
The full form of the JEE Main is the Joint Entrance Exam Main (JEE Main). The exam is a national-level entrance test conducted as a computer-based test for admission to B.E/B.Tech/B.Planning/B.Arch courses. The authorities conduct the JEE Main exam for 2 papers. Paper 1 is conducte...
Herein, the Ugi four-component reaction of perfluorinated acids is utilized to establish an exemplary database consisting of 130 commercially available components. Considering all permutations, this combinatorial approach can unambiguously provide 500,000 molecular keys in only one synthetic procedure per ...
We identified Rubicon, an autophagy suppressor, as a key mediator of DNA damage in GCs under OS. Hydrogen peroxide (H2O2) compromised cell viability via DNA damage in the human GC cell line, HGrC1, without affecting autophagic activity. However, autophagy activation increased OS resistance in HG...
Principal-component and hierarchical cluster analyses revealed that Gli1+ cells in BM fibrosis are drastically distinct from Gli1+ cells in homeostasis (Figures 5A and 5B). Statistical analysis revealed that 1,597 genes were differentially expressed between the Gli1+ cells in control conditions and ...
[13]. Based on the systematic evaluation, the ComBat function was applied to the data to remove the batch effect between the platforms [14]. Principal component and clustering analysis were used to evaluate whether the batch effect was removed. The data were then normalized by TMM method [15...
Additionally, we validate the value of this approach by demonstrating the role of a novel phosphorylation site in EARLY FLOWERING 4 (ELF4 (S-45)), a key component of a tripartite evening represssor complex (EC) in the Arabidopsis circadian oscillator. EXPERIMENTAL PROCEDURES Plant Material and ...
assay, effects at the level of DNA molecule that could be repaired and thus not necessarily translated in detectable effects on cellular response, can be captured. In our experimental conditions, neither permanent effects nor transient modifications have been elicited by SMF exposures. As a matter ...
1.2 Superoxide dismutase (SOD) as the component of the antioxidative system The amount of ROS present in the cell depends on the activity of both the enzymes that generate them and the enzymes removing them, that is, antioxidant enzymes. Small-molecule antioxidants also play an important role in...