Inspired by biosynthesis, we have investigated the natural product synthesis utilizing electrochemical reactions as the key step, in which anodic oxidation of phenol compounds afford the important frameworks such as diaryl ether, spirodienone, and spiroisoxazoline.doi:10.1002/tcr.202100032...
These key compounds are used to compare the results from earlier studies of model compounds such as, e.g., glucose or cellulose, with biomass degradation in order to identify chemical reaction pathways. Key compounds identified are phenols (phenol and cresols), furfurals, acids (acetic acid, ...
DyP2 has demonstrated high manganese peroxidase activity and lignin depolymerization ability, with broad spectrum of substrate specificity, demonstrating peroxidase activity toward phenol, azo dyes and anthraquinone dyes. Laccase Laccase is a multicopper oxidase that is widely used in the biological industry...
N2A cells transfected with PIEZOmScarlet constructs were prepared as described above and imaged in phenol-red free medium. Time-lapse recordings were preprocessed in Fiji (v2.3.0/1.53f) before track analysis, with a background subtraction and a bleach correction (Histrogram matching). PIEZO...
The phenolic substances detected in Daqu mainly included phenol, p-cresol, 2-methylphenol, 2-methoxyphenol, 2,6-dimethoxyphenol, 2-methoxy-4-vinylphenol, and 4-ethyl-2-methoxyphenol. Phenolic compounds have a high odor intensity and are important aroma components of Daqu. For example, 4-ethyl-2...
ER-phagy was induced with media starvation using EBSS with calcium, magnesium, and phenol red (Invitrogen e10043). For EATR and CCER assays, cells were plated 48 hours prior to EBSS treatment. EATR expression is induced using 4 μg/ml doxycycline 24hr prior to starvation. Unless otherwise ...
Due to the plethora natural products made by Streptomyces, the regulation of its metabolism are of great interest, whereas there is a lack of detailed understanding of the role of posttranslational modifications (PTM) beyond traditional transcriptional r
The oxygen in phenol is part of a larger “pi system”, and the electron density can be distributed throughout the aromatic ring via resonance. (Remember: lower charge density = more stability). Let’s apply this to amines. By analogy, we should also expect that aminobenzene (“aniline”...
Cy5-PEG-NPs Uptake Study by flow cytometry. Cells were washed with phosphate buffered saline (PBS 1X), detached with trypsin/EDTA (0.25%, phenol red) (Gibco), counted and diluted with media to a final concentration of 105 cells per mL. 500 μL of each cell line suspension were ...
The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many embodiments, it will be preferable to include isotonic agents, for example, sugars or ...