The enzyme, which had acetyl-CoA:ACP acyltransferase (ACAT) activity, was resolved from a second, specific, ACAT protein. The KASIII enzyme had a derived molecular mass of 42kDa (from its cDNA sequence) and operated as a dimer. Its enzymological characteristics were similar to those of two...
TheA. tumefaciensC58 Atu2127 gene encodes a predicted type III acyl-CoA transferase (annotated as CaiB) that seemed a likely candidate for conversion of glutarate to glutaryl-CoA via transfer of CoA from succinyl-CoA. The possibility that CaiB might provide glutaryl-CoA for the BioZ reaction w...
The catalysis requires activated substrates, which is achieved by a thioester linkage to the 4’ phosphopantethein prosthetic group of ACP, an ACP domain or coenzyme A (CoA). As illustrated in Figure 1 a condensation reaction is tripartite, (i) An activated primer is transferred to a cysteine...
EC number 2.3.1.39 Systematic name malonyl-CoA: [acyl-carrier-protein] S-malonyltransferase Recommended name D Schomburg,Dr. Drte Stephan - Springer Berlin Heidelberg 被引量: 0发表: 1996年 Solubilization and partial purification of constituents of acyl-CoA elongase from Lunaria annua 3-Oxoacyl-(Ac...
However, PKSs suffer from complicated architecture, energy inefficiencies, complex regulation, and competition with essential metabolic pathways for extender unit malonyl-CoA, all combining to limit the flux of polyketide biosynthesis. Here we show that certain thiolases, which we term polyketoacyl-CoA...
(CLF) activity; malonyl-CoA:ACP acyltransferase (MAT) activity; and 4′-phosphopantetheinyl transferase (PPTase) activity; (c) a nucleic acid sequence encoding an amino acid sequence that is at least about 65% identical, and more preferably at least about 75% identical, and more preferably at...
This requires the previous formation of malonyl-CoA from acetyl-CoA and bicarbonate by acetyl-CoA carboxylase (ACC) before the malonyl group of malonyl-CoA is transferred from CoA to acyl carrier protein (ACP) by the malonyl-CoA:ACP malonyltransferase (MCMT). Then, the first condensation is ...
The abbreviations used are: STPK, Ser/Thr protein kinase; ACP, acyl carrier protein; FAS-I, eukaryotic type I fatty acid synthase; FAS-II, prokaryotic type II fatty acid synthase; PDIM, phthiocerol dimycocerosate; GST, glutathione S-transferase; KAS, β-ketoacyl-ACP synthase; MS, mass ...
This method utilizes three CoA biosynthetic enzymes (CoaA, CoaD, CoaE) to form CoA analogues and a phosphopantetheinyl transferase (PPTase, Sfp) to load them onto apo-AcpP, resulting in crypto-AcpP. Final reaction concentrations: 1 mg·mL–1 apo-AcpP, 0.04 mg·mL–1 Sfp, 0.01 ...
One such important protein is β-ketoacyl acyl carrier protein (ACP) synthase (KAS III), which produces acetoacyl ACP from malonyl-ACP using acetyl-Coenzyme A(CoA) as a substrate in the initiation step of the FAS elongation cycle. Hence, KAS III represents an effective target for novel ...