Full size image The developmental phases in the growth cycle of V. dahliae include hyphal growth, production of conidia, microsclerotia precursor formation, and microsclerotia maturation [41]. To explore whether VdNuo1 is involved in these developmental phases, we analyzed the gene expression profi...
To verify the presumed role of the GGC1274-1276 codon in translation initiation, we generated various mutations within this codon in the full-length (4 011 bp) ACAT1 cDNA K1 (Figure 1A, top panel) and performed western blot analyses using an anti-ACAT1 antibody that recog- nizes both 50...
Molecular masses were calculated for full-length proteins expressed from constructs. 1-d Molecular masses were calculated for proteins in which the N-terminal signal peptide of Sep15 was cleaved between Ala28 and Phe29. DISCUSSION In this report, we described the association between Sep15 and UG...
SNAP-25 antibodies reacted with the full-length protein (32 kDa) as well as a breakdown product (28 kDa) present in the starting material (Fig. 4 B, lane 2). The identity of the breakdown product was confirmed by N-terminal protein sequencing, corresponding to a truncation of the N ...
Full size image Full size image Reference-free 2D classification of gaussian-picked particles yielded featureful 2D class averages representing numerous views of iPKAcwherein the N- and C-terminal lobes of the complex, as well as secondary-structural elements, could be clearly discerned. Consistent...
Full size image Under these assumptions, we analysed the contacts between the validated ligand structure and the 8 kDa domain atoms. The aromatic groups of pamoic acid probably form favorable hydrophobic interactions with the main amino-acids of the site, such as Tyr39, Ala42, Gly64 and Gly66...
- 《Molecular Biology of the Cell》 被引量: 77发表: 2011年 Interaction Studies between the Chloroplast Signal Recognition Particle Subunit cpSRP43 and the Full-length Translocase Alb3 Reveal a Membrane-embedded Bin... Posttranslational targeting of the light-harvesting chlorophyll a,b-binding ...
A clear genotype/phenotype relationship has not been estab- lished, nor is it definitively known that alterations in transcription of Thap1 downstream targets are respon- sible for DYT6, as Thap1 may have other, yet to be iden- tified functions. In order to study the biology of endogenous...
Lin,Wei,Fullner,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 373发表: 1999年 Structural and functional relationships among the RTX toxin determinants of Gram‐negative bacteria The RTX (repeats in toxin) cytolytic toxins r represent a family...
In addition to substantially full-length proteins, the invention also includes fragments (e.g., antigenic fragments) of the 120 kDa immunoreactive protein of Ehrlichia canis (SEQ ID No: 7). As used herein, "fragment," as applied to a polypeptide, will ordinarily be at least 10 residues, mo...