The primary rabbit polyclonal antibody directed against human KCNQ1 (sc-20816; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA) was incubated overnight at a 1 : 200 dilution at 4 °C, followed by incubation with anti-rabbit secondary antibodies for 30 min at room temperature (...
Sections were blocked with 10% normal goat serum/TBS/1% BSA for 25 minutes at room temperature and subsequently incubated overnight at 4°C with the primary antibody (0.004 g/L). IgG antibody (Santa Cruz, normal rabbit IgG, # sc-2027) negative controls (0.004 g/L) and no-antibody (...
immunoreactivity was revealed using an Alexa-Fluor 488-conjugated secondary antibody (1:400; Life Technologies, reference Molecular Probes, Invitrogen, A21206) for 90-min at RT. After PBS washes, sections were incubated with 0.02% Hoechst (H3569; Invitrogen) in PBS for 15-min at RT and cover...
The myocytes were fixed in 4% paraformaldehyde, permeabilized by 0.2% Triton X-100, and probed with the following primary/secondary antibody pairs: Q1 goat pAb (C-20, Santa Cruz)/Alexa568-conjugated anti-goat and HA mouse mAb/Alexa488-conjugated anti-mouse. The nuclei were stained by 4′,...