qPCR was performed using 4.5 μl of 1:200 diluted cDNA (or 1:20, or 1:2000 in the primer efficiency calculations), 5 μl of SYBR Green (catalogue no. 4367659, Applied Biosystems, Foster City, CA), and 0.5 μl of 5 μm primer. qPCR was run on the StepOnePlus system (Applied ...
This useful book will be of benefit topost-16 students who want topractise the different type ofchemical calculations met at AS-level and beyond.The contentis divided into five parts (22chapters) and these are clearlyexplained in the introductory pages,eg part one - Getting cosy withnumbers, ...